New Phosphated Polymers Prevent Microbial Colonization

新型磷酸盐聚合物可防止微生物定殖

• 批准号: 7407388
• 负责人: Andrew R Dentino
• 金额: $ 18.17万
• 依托单位: MARQUETTE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7407388
• 关键词: 2-hydroxyethyl methacrylate; Adherence; Adhesions; Adsorption; Affinity; Animal Model; Biological; Candida albicans; Cell Line; Cells; Characteristics; Charge; Clinical; Clinical Treatment; Communicable Diseases; Condition; Defensins; Dental Materials; Dentsply; Denture Bases; Denture Stomatitis; Dentures; Disinfection; Edentulous Mouth; Elasticity; Elderly; Electrostatics; Epithelial Cells; Ethylene Glycols; Exhibits; Fibroblasts; Generations; Gingiva; Goals; Guidelines; Hardness; Human; In Vitro; Infection; Lead; Mechanics; Methacrylates; Oral; Oral health; Organism; Patients; Peptides; Phase; Plant Resins; Polymers; Polymethyl Methacrylate; Population; Porosity; Prevention; Property; Prosthesis; Research; Research Project Grants; Residual state; Resistance; Safety; Saliva; Salivary; Specimen; Standards of Weights and Measures; Stomatitis; Streptococcus; Streptococcus gordonii; Streptococcus mutans; Streptococcus oralis; Streptococcus sanguis; Surface; Surface Properties; Suspension substance; Suspensions; Techniques; Testing; Tissues; Tooth structure; antimicrobial; base; biomaterial compatibility; commercial application; cytotoxic; cytotoxicity; ethylene glycol; histatin 5; histidine-rich proteins; improved; inorganic phosphate; microbial; microbial colonization; microbicide; monomer; oral bacteria; pathogen; polymerization; prevent; research study; tooth surface; water solubility

DESCRIPTION (provided by applicant): The goal of this research project is to develop a new generation of denture-base polymers that could control microbial colonization and prevent denture-induced stomatitis. These new polymers will be synthesized by the incorporation of phosphate groups within poly (methyl methacrylate) [PMMA] to prevent denture infection. Phosphate groups in the new polymers will provide the required anionic surface for the salivary cationic antimicrobials to be selectively adsorbed onto the denture surface. This will result in the formation of a defense pellicle layer on the denture surface. The currently available PMMA, due to the absence of ionic surface and the defense denture pellicle component, does not inhibit microbial colonization This research project involves: 1) Synthesis of phosphated PMMAs containing varied amounts of phosphate groups and non-phosphated PMMA and characterization of polymers; 2) Assessment of the efficacy of this polymer to adsorb and desorb salivary antimicrobials using histatin 5 and a defensin fragment; 3)Determination of the microbicidal activity of antimicrobials adsorbed phosphated polymers using Candida albicans, Streptococcus Sanguis, Streptococcus oralis, Streptococcus gordonii and Streptococcus mutans; 4) Delineation of the physical and mechanical properties of polymers that exhibit high affinity for salivary antimicrobials, and inhibit microbial adhesion to the polymer surface following the ADA/ISO guidelines; 5) Assessment of the in vitro cytotoxicity of polymer specimens against a human oral epithelial cell line, human primary gingival fibroblast cells, and the standard L-929 cell line to ascertain the safety requirements for Dental materials. Relevance: This proposed research will identify new, safe and affordable phosphated PMMA polymers that could serve as denture-base or denture base lining materials for the prevention and treatment of denture infection. These new polymers will exhibit physical, mechanical and biological properties comparable to those of normal oral surfaces, and will have significant impact on the expenses involved in the treatment of denture-induced infection associated with the existing PMMA polymer. The use of these new polymers could improve the oral health of a significant population of prosthesis users. The phosphated PMMA polymers could have great potential for clinical and commercial applications.

描述（由申请人提供）：本研究项目的目标是开发新一代义齿基托聚合物，可以控制微生物定植并预防义齿诱导的口腔炎。这些新的聚合物将通过在聚甲基丙烯酸甲酯[PMMA]中引入磷酸基团来合成，以防止义齿感染。新聚合物中的磷酸基团将为唾液阳离子抗菌剂提供所需的阴离子表面，以选择性地吸附到义齿表面上。这将导致在义齿表面上形成防御薄膜层。目前可用的PMMA，由于缺乏离子表面和防御义齿薄膜组件，不能抑制微生物定植。本研究项目包括：1）合成含有不同量磷酸基团的磷酸化PMMA和非磷酸化PMMA，并表征聚合物; 2）使用组胺素5和防御素片段评估该聚合物吸附和解吸唾液抗菌剂的功效; 3）使用白色念珠菌（Candida albicans）、血链球菌（Streptococcus Sanguis）、口腔链球菌（Streptococcus oralis）、戈登链球菌（Streptococcus gordonii）和变形链球菌（Streptococcus mutans）测定吸附有抗菌剂的磷酸化聚合物的杀微生物活性; 5）评估聚合物样品对人口腔上皮细胞系、人原代牙龈成纤维细胞和标准L-929细胞系的体外细胞毒性，以确定牙科材料的安全要求。相关性：这项拟议的研究将确定新的，安全的和负担得起的磷酸化PMMA聚合物，可作为义齿基托或义齿基托衬里材料，用于预防和治疗义齿感染。这些新的聚合物将表现出与正常口腔表面相当的物理、机械和生物学特性，并且将对与现有PMMA聚合物相关的义齿诱导感染的治疗费用产生重大影响。使用这些新的聚合物可以改善大量假体使用者的口腔健康。磷酸化的PMMA聚合物具有巨大的临床和商业应用潜力。