Robust Self-Assembled Monolayers for Improved Hemocompatibility

项目摘要/摘要植入式血液接触医疗器械，如左心室辅助装置（VADs），会给患者带来血栓形成风险。这些风险在儿童心室辅助装置的小血管中加剧。虽然市面上的血液相容性涂层还不能提供完全惰性的表面来抵抗血块的形成，但最近的研究表明，聚乙二醇（PEG）的自组装单层（sam）在这方面很有希望。不幸的是，这些涂层与表面的结合很弱，脱附会迅速降低性能。该项目的目的是形成惰性的共价键聚乙二醇单层膜，这种单层膜不会降解，并将延长vad和其他可植入设备的使用寿命。这将通过一种基于超临界二氧化碳的新型SAM沉积方法来实现，该方法可以产生具有高接枝密度和出色均匀性的坚固单层。涂层将使用表面分析方法进行全面表征。蛋白质吸附和细胞粘附将在控制流动条件下使用全血测定来探测。增强血液相容性的坚固自组装单层膜血栓形成的风险在心室辅助装置中是显著的，特别是在儿科装置的小血液导管中。这项工作旨在通过提供与钛和氧化铝基体的共价化学键来推进有前途的聚乙二醇（PEG）涂层。共价键合将提高聚乙二醇涂层在流动诱导剪切载荷下的耐久性。当通过超临界二氧化碳溶液沉积时，聚乙二醇将与硅烷官能团连接并形成高度均匀的自组装单层（SAM）。

DESCRIPTION (provided by applicant): Robust Self-Assembled Monolayers for Improved Hemocompatibility Principal Investigator: Brink, Damon Project Summary / Abstract Implantable blood-contacting medical devices such as left ventricular assist devices (VADs) pose thrombosis risks to patients. These risks are exacerbated in the small blood conduits of pediatric ventricular assist devices. While commercially available hemocompatible coatings do not yet provide fully inert surfaces that resist clot formation, recent research suggests that self-assembled monolayers (SAMs) of poly(ethylene glycol) (PEG) hold promise in this regard. Unfortunately, these coatings are only weakly bonded to the surface and desorption quickly degrades performance. The aim of this project is to form inert, covalently-bonded PEG monolayers that do not degrade and will extend the usable life of VADs and other implantable devices. This will be accomplished using a novel SAM deposition method based on supercritical carbon dioxide which produces robust monolayers having high graft density and outstanding uniformity. The coatings will be fully characterized using surface analysis methods. Protein adsorption and cellular adhesion will be probed using whole blood assays under controlled flow conditions. Robust Self-Assembled Monolayers for Improved Hemocompatibility Principal Investigator: Brink, Damon Project Narrative Risks of thrombosis formation are significant in ventricular assist devices - particularly in the small blood conduits of pediatric devices. This work seeks to advance the promising poly(ethylene glycol) (PEG) coatings by providing a covalent chemical bond to titanium and alumina substrates. Covalent bonding will enhance the durability of the PEG coatings in the presence of flow induced shear loads. The PEG will be linked with silane functional groups and form a highly uniform self-assembled monolayer (SAM) when deposited via a solution of supercritical carbon dioxide.