800 MHz NMR Spectrometer for High-End NMR Structural Studies

用于高端 NMR 结构研究的 800 MHz NMR 波谱仪

• 批准号: 7125694
• 负责人: JOHN Hackett BUSHWELLER
• 金额: $ 200万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7125694
• 关键词: detergents; magnetism; membrane proteins; noise; performance; solubility; solutions; university

DESCRIPTION (provided by applicant): Eight faculty members at the University of Virginia are using high-resolution multi-dimensional NMR in their studies of biopolymers. These investigators are all well-funded with grants from the NIH and other sources. The use of NMR to elucidate biomolecular structure and function is an integral part of their NIH funded research. Due to the challenges of the systems being worked on by these investigators, including limitations in solubility and/or stability, lack of dispersion (spread), and a focus on large systems such as integral membrane proteins solubilized in detergents, this group has identified a distinct need for higher field NMR instrumentation on campus. To this end, we are requesting funding for an 18.8 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at the University of Virginia. This spectrometer will be used for high-resolution multi- dimensional NMR experiments with labeled (15N, 13C and 15N, 13C, 2H) biopolymers in solution as well as in the presence of various alignment media. In order to accommodate the requirements of the installation site, the magnet will be actively shielded to minimize the effects of the stray field on neighboring laboratories. The spectrometer will be equipped with 4 radiofrequency (RF) channels with one dual fullband RF system capable of operating at the 1H frequency and lower and one dual broadband RF system capable of operating at the 15N to 31P frequency range. The spectrometer will also be equipped with a He-cooled triple resonance 1H-detection cryoprobe equipped with a single-axis pulsed-field gradient capability. This probe provides a dramatic improvement in the signal-to-noise which is essential for the majority of the user applications. The 800 MHz NMR spectrometer will provide dramatic enhancements in three critical performance criteria that are essential for the research efforts of our user group: 1) The 800 MHz NMR spectrometer provides a substantial increase in the signal-to-noise (S/N). 2) The 800 MHz NMR spectrometer provides a substantial increase in the dispersion (spread) of NMR signals. 3) The 800 MHz NMR spectrometer enhances the performance of TROSY-based experiments.

描述（由申请人提供）：弗吉尼亚大学的八名教员正在使用高分辨率多维 NMR 来研究生物聚合物。这些研究人员都获得了美国国立卫生研究院和其他来源的充足资助。使用 NMR 来阐明生物分子结构和功能是 NIH 资助的研究的一个组成部分。由于这些研究人员正在研究的系统面临挑战，包括溶解度和/或稳定性的限制、缺乏分散性（扩散）以及对大型系统（例如溶解在洗涤剂中的整体膜蛋白）的关注，该小组已经确定了校园内对更高场核磁共振仪器的明显需求。为此，我们请求资助在弗吉尼亚大学安装一台 18.8 T (800 MHz 1H) 核磁共振 (NMR) 波谱仪。该光谱仪将用于在溶液中以及在存在各种对准介质的情况下对标记（15N、13C 和 15N、13C、2H）生物聚合物进行高分辨率多维 NMR 实验。为了适应安装地点的要求，磁铁将被主动屏蔽，以尽量减少杂散场对邻近实验室的影响。该光谱仪将配备 4 个射频 (RF) 通道，其中 1 个双全频带 RF 系统能够在 1H 及更低频率下运行，1 个双宽带 RF 系统能够在 15N 至 31P 频率范围下运行。该光谱仪还将配备具有单轴脉冲场梯度功能的 He 冷却三重共振 1H 检测冷冻探针。该探头显着改善了信噪比，这对于大多数用户应用至关重要。 800 MHz NMR 波谱仪将显着增强三个关键性能标准，这对于我们用户组的研究工作至关重要：1) 800 MHz NMR 波谱仪显着提高了信噪比 (S/N)。 2) 800 MHz NMR 波谱仪显着提高了 NMR 信号的色散（扩展）。 3) 800 MHz NMR 波谱仪增强了基于 TROSY 的实验性能。

项目成果

Membrane targeting of TIRAP is negatively regulated by phosphorylation in its phosphoinositide-binding motif.

TIRAP 的膜靶向受到其磷酸肌醇结合基序的磷酸化的负向调节。

• DOI: 10.1038/srep43043
• 发表时间: 2017
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Zhao,Xiaolin;Xiong,Wen;Xiao,Shuyan;Tang,Tuo-Xian;Ellena,JeffreyF;Armstrong,GeoffreyS;Finkielstein,CarlaV;Capelluto,DanielGS
• 通讯作者: Capelluto,DanielGS

Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism.

Tom1 通过耦合折叠和结合机制调节 Tollip 与磷脂酰肌醇 3-磷酸的结合。

• DOI: 10.1016/j.str.2015.07.017
• 发表时间: 2015
• 期刊: Structure (London, England : 1993)
• 作者: Xiao,Shuyan;Brannon,MaryK;Zhao,Xiaolin;Fread,KristenI;Ellena,JeffreyF;Bushweller,JohnH;Finkielstein,CarlaV;Armstrong,GeoffreyS;Capelluto,DanielGS
• 通讯作者: Capelluto,DanielGS