800 MHZ NMR SPECTROMETER FOR HIGH-END NMR STRUCTURAL STUDIES

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：弗吉尼亚大学的八名教师正在使用高分辨率多维NMR研究生物聚合物。这些研究人员都得到了NIH和其他来源的赠款。使用核磁共振来阐明生物分子的结构和功能是他们NIH资助研究的一个组成部分。由于这些研究人员正在研究的系统面临的挑战，包括溶解度和/或稳定性的限制，缺乏分散性（扩散），以及对大型系统的关注，例如溶解在洗涤剂中的膜蛋白质，该小组已经确定了对校园内更高场NMR仪器的明显需求。为此，我们正在申请资金，用于在弗吉尼亚大学安装一台18.8 T（800 MHz 1H）核磁共振（NMR）光谱仪。该光谱仪将用于溶液中标记的（15 N，13 C和15 N，13 C，2 H）生物聚合物以及各种配向介质存在下的高分辨率多维NMR实验。为了适应安装现场的要求，磁体将被主动屏蔽，以最大限度地减少杂散场对邻近实验室的影响。光谱仪将配备4个射频（RF）通道，其中一个双全频带RF系统能够在1H频率和更低频率下工作，另一个双宽带RF系统能够在15 N至31 P频率范围内工作。光谱仪还将配备氦冷却三重共振1H检测冷冻探针，该探针配备有单轴脉冲场梯度能力。该探头显著改善了信噪比，这对于大多数用户应用至关重要。800 MHz NMR光谱仪将在三个关键性能标准方面提供显着的增强，这对我们的用户组的研究工作至关重要：1）800 MHz NMR光谱仪提供了信噪比（S/N）的大幅增加。2)800 MHz NMR光谱仪提供了NMR信号的色散（扩展）的显著增加。3)800 MHz NMR光谱仪增强了基于TROSY的实验的性能。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Eight faculty members at the University of Virginia are using high-resolution multi-dimensional NMR in their studies of biopolymers. These investigators are all well-funded with grants from the NIH and other sources. The use of NMR to elucidate biomolecular structure and function is an integral part of their NIH funded research. Due to the challenges of the systems being worked on by these investigators, including limitations in solubility and/or stability, lack of dispersion (spread), and a focus on large systems such as integral membrane proteins solubilized in detergents, this group has identified a distinct need for higher field NMR instrumentation on campus. To this end, we are requesting funding for an 18.8 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at the University of Virginia. This spectrometer will be used for high-resolution multi- dimensional NMR experiments with labeled (15N, 13C and 15N, 13C, 2H) biopolymers in solution as well as in the presence of various alignment media. In order to accommodate the requirements of the installation site, the magnet will be actively shielded to minimize the effects of the stray field on neighboring laboratories. The spectrometer will be equipped with 4 radiofrequency (RF) channels with one dual fullband RF system capable of operating at the 1H frequency and lower and one dual broadband RF system capable of operating at the 15N to 31P frequency range. The spectrometer will also be equipped with a He-cooled triple resonance 1H-detection cryoprobe equipped with a single-axis pulsed-field gradient capability. This probe provides a dramatic improvement in the signal-to-noise which is essential for the majority of the user applications. The 800 MHz NMR spectrometer will provide dramatic enhancements in three critical performance criteria that are essential for the research efforts of our user group: 1) The 800 MHz NMR spectrometer provides a substantial increase in the signal-to-noise (S/N). 2) The 800 MHz NMR spectrometer provides a substantial increase in the dispersion (spread) of NMR signals. 3) The 800 MHz NMR spectrometer enhances the performance of TROSY-based experiments.