LSM 510 CONFOCAL MICROSCOPE: BRAIN

LSM 510 共焦显微镜：大脑

• 批准号: 7335224
• 负责人: ROBERT B GIBBS
• 金额: $ 10.37万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335224
• 关键词: brain; schools

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This request is for funds to purchase a Zeiss 510 meta confocal microscope. This instrument will be housed on the 10th floor of Salk Hall in the University of Pittsburgh School of Pharmacy. Basic and clinical research within the School of Pharmacy has grown tremendously over the past six years. During the past four years, the School of Pharmacy has been rated among the top 10 in NIH funding among all Schools of Pharmacy in the nation. Despite this success, the School is relatively equipment poor and is forced in many cases to rely on major equipment in other schools. Not only does this create difficulties for existing personnel, but also presents obstacles in recruiting highly qualified research faculty. The purpose of this request is to provide the School of Pharmacy with its own confocal instrument to meet the critical needs of its research faculty. Specifically, the School currently is in need of a four-laser, spectral, confocal microscope, equipped for live cell imaging. Six NIH-funded investigators within the School have identified a need for such equipment. Most of this work falls within the realm of new drug development (particularly the development of anti-cancer agents and gene therapy methodologies) and focuses on following the uptake and trafficking of molecules and drugs within cells and tissues. Currently, the School of Pharmacy has no confocal instrument. Instruments are available elsewhere within the University, primarily at the University's Center for Biologic Imaging (CBI). Unfortunately, CBI is not able to provide sufficient access to accommodate our needs. CBI resources are in very high demand, and School of Pharmacy personnel have the lowest priority with regard to access. For example, School of Pharmacy personnel have no access on evenings or weekends, and are limited to 3-hour time slots, when slots are available. Often School of Pharmacy personnel must wait 1-2 weeks before getting access to an instrument, and during the access time available are not able to analyze and collect all of their data. This lack of available instrument time is extremely frustrating and limits the pace of our research. Acquisition of the requested instrument will eliminate this problem, and will provide the School of Pharmacy with a greatly needed resource.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：此请求是要购买Zeiss 510 Meta共聚焦显微镜的资金。该乐器将安置在匹兹堡大学药学院的Salk Hall 10楼。在过去的六年中，药学院内的基础和临床研究已经大大增加。在过去的四年中，药学院在全国所有药房中的NIH资金中都被评为十大。尽管取得了成功，但学校的设备相对较差，并且在许多情况下被迫依靠其他学校的主要设备。这不仅给现有人员带来了困难，而且在招募高素质的研究学院方面存在障碍。该请求的目的是为药学院提供自己的共聚焦工具，以满足其研究学院的关键需求。具体而言，该学校目前需要四射线器，光谱，共聚焦显微镜，配备了活细胞成像。学校内的六名NIH资助的调查人员已经确定了对这种设备的需求。这项工作的大部分都属于新药开发的领域（尤其是抗癌剂和基因治疗方法的发展），并着重于遵循细胞和组织中分子和药物的摄取和运输。目前，药学院没有共聚焦工具。仪器可在大学其他地方提供，主要在大学的生物成像中心（CBI）。不幸的是，CBI无法提供足够的访问来满足我们的需求。 CBI资源的需求很高，药学院人员在访问方面的优先级最低。例如，药学院工作人员在晚上或周末无法访问，并且有3个小时的时间插槽，当时有3个小时的插槽。通常，药房工作人员必须等待1-2周才能访问乐器，并且在访问时间内无法分析和收集所有数据。缺乏可用的乐器时间非常令人沮丧，并限制了我们研究的步伐。收购所请求的工具将消除此问题，并将为药学院提供非常需要的资源。