HYBRID TRIPLE QUADRUPOLE LINEAR ION TRAP MASS SPECTROMETER

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：本申请的目的是通过获得混合三重四极线性离子阱质谱仪来加强我们在年龄相关性、炎症和感染性疾病方面的研究计划。新LC-MS/MS仪器的获得将使俄勒冈州州立大学（OSU）环境健康科学中心（EHSC）和健康老龄化研究中心（CHAiR）附属的六名主要研究人员的主要用户组受益。该应用背后的NIH资助的驱动生物项目涉及生物氧化剂与蛋白质，脂质，维生素和膳食植物化学物质之间相互作用的基础研究。他们的重点是（1）过氧亚硝酸盐介导的酪氨酸硝化在肌萎缩侧索硬化症中的作用，（2）氧化应激对心脏线粒体衰老中神经酰胺周转的作用，（3）识别用于预防前列腺癌的新型膳食植物化学物质，（4）脂质过氧化介导的线粒体蛋白损伤在心脏衰老中的作用，（5）脂质过氧化产物的抗坏血酸化在预防动脉粥样硬化形成中的作用;（6）维生素E及其代谢产物对药物和其他外源性物质的肝脏代谢的影响。所要求的仪器还将支持一个二级用户组的五个NIH资助的教师和五个NIH资助的偶尔用户在俄勒冈州立大学。与我们现有的PE Sciex API III Plus质谱仪相比，所要求的仪器的优点是：（1）定量分析的灵敏度显著更高，（2）更好的碰撞池，允许在单次色谱运行期间定量复杂生物基质中的数百种分析物，（3）由于不同的真空泵送系统而增加的样品通量，该系统不需要过夜回收，（4）更现代化的数据采集和数据处理计算机系统。线性离子阱的增强子离子扫描的独特功能可以为血浆、细胞和细胞器提取物中生物小分子及其代谢产物的鉴定提供丰富的结构信息，这是主要驱动生物项目的重要焦点。负离子和正离子模式之间的快速切换，结合前体离子，中性损失和混合仪器上的产物离子扫描，将为三个NIH资助的研究人员的研究活动中绘制磷酸化位点和蛋白质的其他翻译后修饰提供一个非常强大的工具。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The objective of this application is to strengthen our programs of research in age-related, inflammatory and infectious diseases by acquiring a hybrid triple quadrupole linear ion trap mass spectrometer. The acquisition of a new LC-MS/MS instrument will benefit a main user group of six principal investigators affiliated with the Environmental Health Sciences Center (EHSC) and the Center for Healthy Aging Research (CHAiR) at Oregon State University (OSU). The NIH-funded driving biological projects behind this application involve fundamental studies on the interaction between biological oxidants and proteins, lipids, vitamins, and dietary phytochemicals. They are focused on (1) the role of peroxynitrite-mediated tyrosine nitration in amyotrophic lateral sclerosis, (2) the role of oxidative stress on ceramide turnover in cardiac mitochondrial aging, (3) the identification of novel dietary phytochemicals for the prevention of prostate cancer, (4) the role of lipid peroxidation-mediated damage to mitochondrial proteins in cardiac aging, (5) the role that ascorbylation of lipid peroxidation products plays in the prevention of atherogenesis, and (6) the effects of vitamin E and its metabolites on hepatic metabolism of drugs and other xenobiotics. The requested instrument will also support a secondary user group of five NIH-funded faculty and five NIH-funded occasional users at OSU. The advantages of the requested instrument compared to our existing PE Sciex API III Plus mass spectrometer are (1) the significantly higher sensitivity for quantitative analysis, (2) a better collision cell that allows quantification of hundreds of analytes in complex biological matrices during a single chromatographic run, (3) increased sample throughput due to a different vacuum pumping system that needs no overnight recycling, and (4) a more modern data acquisition and data processing computer system. The unique feature of enhanced product ion scanning of the linear ion trap can provide a wealth of structural information for the identification of small biological molecules and their metabolites in extracts of plasma, cells and cell organelles, which is an important focus of the main driving biological projects. Rapid switching between the negative and positive ion modes in combination with precursor ion, neutral loss, and product ion scanning on the hybrid instrument will provide an extremely powerful tool for mapping phosphorylation sites and other post-translational modifications of proteins in the research activities of three NIH-funded investigators.