HYBRID TRIPLE QUADRUPOLE LINEAR ION TRAP MASS SPECTROMETER

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：本申请的目标是通过获得混合型三重四极线型离子陷阱质谱仪，加强我们在年龄相关、炎症和传染病方面的研究计划。购买新的LC-MS/MS仪器将使环境健康科学中心(EHSC)和俄勒冈州立大学(OSU)健康老龄化研究中心(主席)附属的六名主要研究人员的主要用户群体受益。NIH资助的推动这一应用的生物学项目涉及生物氧化剂与蛋白质、脂类、维生素和膳食植物化学物质之间相互作用的基础研究。它们集中在(1)过氧亚硝酸盐介导的酪氨酸硝化在肌萎缩侧索硬化症中的作用，(2)氧化应激对神经酰胺更新在心脏线粒体老化中的作用，(3)用于预防前列腺癌的新的饮食植物化学物质的鉴定，(4)脂质过氧化介导的线粒体蛋白质损伤在心脏老化中的作用，(5)脂质过氧化产物的抗坏血酸在防止动脉粥样硬化中的作用，以及(6)维生素E及其代谢产物对药物和其他外源生物物质的肝脏代谢的影响。申请的仪器还将支持俄亥俄州立大学由五名NIH资助的教职员工和五名NIH资助的临时用户组成的二级用户群体。与我们现有的PE Sciex API III Plus质谱仪相比，所要求的仪器具有以下优势：(1)定量分析的灵敏度显著提高，(2)更好的碰撞池，可在一次色谱运行中对复杂生物基质中的数百种分析物进行定量，(3)由于不需要隔夜回收的不同真空抽气系统，增加了样品吞吐量，以及(4)更现代化的数据采集和数据处理计算机系统。线性离子陷阱增强产物离子扫描的独特特性可以为鉴定血浆、细胞和细胞器提取物中的生物小分子及其代谢物提供丰富的结构信息，这是主要推动生物计划的重要焦点。在混合仪器上快速切换负离子和正离子模式，结合前体离子、中性损失和产物离子扫描，将为绘制三个由NIH资助的研究人员的研究活动中的蛋白质磷酸化位点和其他翻译后修饰提供极其强大的工具。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The objective of this application is to strengthen our programs of research in age-related, inflammatory and infectious diseases by acquiring a hybrid triple quadrupole linear ion trap mass spectrometer. The acquisition of a new LC-MS/MS instrument will benefit a main user group of six principal investigators affiliated with the Environmental Health Sciences Center (EHSC) and the Center for Healthy Aging Research (CHAiR) at Oregon State University (OSU). The NIH-funded driving biological projects behind this application involve fundamental studies on the interaction between biological oxidants and proteins, lipids, vitamins, and dietary phytochemicals. They are focused on (1) the role of peroxynitrite-mediated tyrosine nitration in amyotrophic lateral sclerosis, (2) the role of oxidative stress on ceramide turnover in cardiac mitochondrial aging, (3) the identification of novel dietary phytochemicals for the prevention of prostate cancer, (4) the role of lipid peroxidation-mediated damage to mitochondrial proteins in cardiac aging, (5) the role that ascorbylation of lipid peroxidation products plays in the prevention of atherogenesis, and (6) the effects of vitamin E and its metabolites on hepatic metabolism of drugs and other xenobiotics. The requested instrument will also support a secondary user group of five NIH-funded faculty and five NIH-funded occasional users at OSU. The advantages of the requested instrument compared to our existing PE Sciex API III Plus mass spectrometer are (1) the significantly higher sensitivity for quantitative analysis, (2) a better collision cell that allows quantification of hundreds of analytes in complex biological matrices during a single chromatographic run, (3) increased sample throughput due to a different vacuum pumping system that needs no overnight recycling, and (4) a more modern data acquisition and data processing computer system. The unique feature of enhanced product ion scanning of the linear ion trap can provide a wealth of structural information for the identification of small biological molecules and their metabolites in extracts of plasma, cells and cell organelles, which is an important focus of the main driving biological projects. Rapid switching between the negative and positive ion modes in combination with precursor ion, neutral loss, and product ion scanning on the hybrid instrument will provide an extremely powerful tool for mapping phosphorylation sites and other post-translational modifications of proteins in the research activities of three NIH-funded investigators.