Acquisition of timsTOF Pro for Complex Lipidomic Analyses

摘要 佛罗里达大学的质谱研究和教育中心（MSREC）是 提交一份新的提案，要求为购买布鲁克timsTOF Pro捕获离子提供资金 将安装四极杆飞行时间质谱仪系统， 在佛罗里达大学的MSREC中运行。我们要求这台仪器专门用于 用于各种研究项目的复杂脂质的“4D-Lipidomicsä”分析。这个布鲁克 timsTOF Pro LC-MS将为PASEF MS/MS增加超高分辨率捕获离子迁移率（TIMS） UF NIH资助的调查人员的能力，目前在东南地区和 佛罗里达的北部/中部地区。MSREC为UF校园内的150多个研究小组提供服务 来自超过25个部门，中心和学院。30多个非UF学术机构和 工业公司也经常使用MSREC核心实验室。 完整的系统将包括timsTOF Pro LC-IM-MS/MS系统， MS和平行累积串联裂解（PASEF），UltiMate 3000 RSLCnano LC系统， Apollo II离子漏斗ESI源，SimLipid企业版学术版，升级到Compass Metaboscape 2021，来自当前许可版本5.0，Library MS-MetaboBase 3.0和Wiley Metlin 质谱数据库。 代谢组学、蛋白质组学和脂质组学领域现在被认为在以下方面发挥着关键作用： 系统生物学，并正在成为诊断测试，新药开发的重点 治疗，个性化医疗，以及对传染病，癌症和代谢的理解 紊乱提出的timsTOF Pro系统将用于测量复杂的脂质混合物 需要超高分辨率离子迁移率质谱进行检测、鉴定， 对这些复杂的混合物进行定量。这些数据将反过来改善与健康相关的结果。 本提案中所列项目的目标，例如： 癌症和其他疾病的治疗;基因间相互作用的基础生物学 动物衰老过程中的环境因素和多重耐药革兰氏阴性杆菌的研究 阴性细菌“超级细菌”和抗生素; SPM与腹主动脉的关系 动脉瘤;了解膜脂质调节GPCR功能的结构机制;以及 开发和理解驱虫药的作用机制。 虽然所要求的仪器能力是针对用户的需要和与健康有关的需要， 为了实现本文所列研究项目的目标，该仪器还将提供实质性的改进， 在数据质量和吞吐量的系统生物学研究人员在UF社区。

ABSTRACT The Mass Spectrometry Research and Education Center (MSREC) at the University of Florida is submitting a new proposal requesting funds for the purchase of a Bruker timsTOF Pro Trapped Ion Mobility Spectrometry Quadrupole Time-of-Flight Mass Spectrometry system to be installed and operated in the MSREC at the University of Florida. We are requesting this instrument specifically for the “4D-Lipidomicsä” analysis of complex lipids for a variety of research programs. This Bruker timsTOF Pro LC-MS will add ultra-high-resolution trapped ion mobility (TIMS) with PASEF MS/MS capabilities to UF NIH-funded investigators that currently is unavailable in the Southeast region and the North/central region of Florida. MSREC serves more than 150 research groups on the UF campus from more than 25 departments, centers, and colleges. More than 30 non-UF academic institutions and industrial companies also frequently utilize the MSREC core lab. The complete system will include the timsTOF Pro LC-IM-MS/MS system with measurements in both MS and Parallel Accumulation Serial Fragmentation (PASEF), an UltiMate 3000 RSLCnano LC system, Apollo II ion funnel ESI source, SimLipid Enterprise Edition Academic, upgrade to Compass Metaboscape 2021 from currently licensed version 5.0, Library MS-MetaboBase 3.0 and Wiley Metlin Mass Spectral Database. The fields of metabolomics, proteomics, and lipidomics are now recognized as playing a critical role in systems biology and are becoming the focus of the development of diagnostic tests, novel drug therapies, personalized medicine, and understanding of infectious diseases, cancer, and metabolic disorders. The proposed timsTOF Pro system will be used for measuring complex mixtures of lipids requiring ultra-high-resolution ion mobility mass spectrometry for detection, identification, and quantitation of those complex mixtures. This data will in turn improve the outcomes of the health related goals of the projects assembled in this proposal, examples including: development of novel therapeutics for cancer and other diseases; fundamental biology of the interactions between genetic factors and environmental factors in the process of animal aging; studying multidrug-resistant Gram- negative bacteria “superbugs” and antibiotics; the relationship between SPMs to abdominal aortic aneurysms; understanding the structural mechanisms by which membrane lipids regulate GPCR function; and developing and understanding the mechanism of anthelmintics. While the requested instruments capabilities are targeted to the needs of the users and health-related goals of the research projects listed herein, the instrument will also provide substantial improvements in data quality and throughput for the community of system biology researchers at the UF.