Acquisition of timsTOF Pro for Complex Lipidomic Analyses

摘要 佛罗里达大学质谱学研究和教育中心(MSREC)是 提交新建议书，申请购买Bruker TimsTOF Pro Traded Ion的资金 将安装迁移率光谱四极杆飞行时间质谱仪系统 在佛罗里达大学的MSREC运作。我们特别要求这台仪器是为了 针对各种研究项目的复杂脂质的“4D-Lipid Economicsä”分析。这个布鲁克 TimsTOF Pro LC-MS将通过PASEF MS/MS增加超高分辨率俘获离子迁移率(TIMS) 为UF NIH资助的调查人员提供目前在东南部地区和 佛罗里达州北部/中部地区。MSREC为密歇根大学校园内的150多个研究小组提供服务 来自超过25个系、中心和学院。30多个非密歇根大学的学术机构和 工业公司也经常使用MSREC核心实验室。 完整的系统将包括TimsTOF Pro LC-IM-MS/MS系统，该系统在两个方面都有测量 MS和并行累加序列分段(PASEF)，终极3000 RSLCNano LC系统， Apollo II离子漏斗ESI源，SimLipid企业版学术版，升级到Compass Metaboscape 2021，来自当前许可的版本5.0、库MS-MetobBase 3.0和Wiley Metline 质谱学数据库。 代谢组学、蛋白质组学和脂质组学领域现在被认为在 而系统生物学正成为诊断试验、新药开发的重点 治疗、个性化医疗以及对传染病、癌症和新陈代谢的了解 精神错乱。拟议的timsTOF Pro系统将用于测量复杂的脂类混合物 需要超高分辨率离子迁移率质谱仪进行检测、鉴定和 这些复杂混合物的定量。这些数据将反过来改善与健康相关的 本提案中汇集的项目的目标，例如：小说的开发 癌症和其他疾病的治疗；基因与基因相互作用的基础生物学 动物衰老过程中的因素和环境因素；耐多药革兰氏菌的研究 阴性菌“超级细菌”与抗生素；SPM与腹主动脉的关系 动脉瘤；了解膜脂调节GPCR功能的结构机制；以及 开发和了解驱虫药的作用机制。 虽然所要求的仪器能力是针对用户的需求和与健康有关的 为了实现本文所列研究项目的目标，该工具还将提供实质性的改进 在数据质量和吞吐量方面，UF的系统生物学研究人员社区。

ABSTRACT The Mass Spectrometry Research and Education Center (MSREC) at the University of Florida is submitting a new proposal requesting funds for the purchase of a Bruker timsTOF Pro Trapped Ion Mobility Spectrometry Quadrupole Time-of-Flight Mass Spectrometry system to be installed and operated in the MSREC at the University of Florida. We are requesting this instrument specifically for the “4D-Lipidomicsä” analysis of complex lipids for a variety of research programs. This Bruker timsTOF Pro LC-MS will add ultra-high-resolution trapped ion mobility (TIMS) with PASEF MS/MS capabilities to UF NIH-funded investigators that currently is unavailable in the Southeast region and the North/central region of Florida. MSREC serves more than 150 research groups on the UF campus from more than 25 departments, centers, and colleges. More than 30 non-UF academic institutions and industrial companies also frequently utilize the MSREC core lab. The complete system will include the timsTOF Pro LC-IM-MS/MS system with measurements in both MS and Parallel Accumulation Serial Fragmentation (PASEF), an UltiMate 3000 RSLCnano LC system, Apollo II ion funnel ESI source, SimLipid Enterprise Edition Academic, upgrade to Compass Metaboscape 2021 from currently licensed version 5.0, Library MS-MetaboBase 3.0 and Wiley Metlin Mass Spectral Database. The fields of metabolomics, proteomics, and lipidomics are now recognized as playing a critical role in systems biology and are becoming the focus of the development of diagnostic tests, novel drug therapies, personalized medicine, and understanding of infectious diseases, cancer, and metabolic disorders. The proposed timsTOF Pro system will be used for measuring complex mixtures of lipids requiring ultra-high-resolution ion mobility mass spectrometry for detection, identification, and quantitation of those complex mixtures. This data will in turn improve the outcomes of the health related goals of the projects assembled in this proposal, examples including: development of novel therapeutics for cancer and other diseases; fundamental biology of the interactions between genetic factors and environmental factors in the process of animal aging; studying multidrug-resistant Gram- negative bacteria “superbugs” and antibiotics; the relationship between SPMs to abdominal aortic aneurysms; understanding the structural mechanisms by which membrane lipids regulate GPCR function; and developing and understanding the mechanism of anthelmintics. While the requested instruments capabilities are targeted to the needs of the users and health-related goals of the research projects listed herein, the instrument will also provide substantial improvements in data quality and throughput for the community of system biology researchers at the UF.