Protein Expression & Proteomics Resource

蛋白质表达

• 批准号: 7514626
• 负责人: Dean P Edwards
• 金额: $ 11.86万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7514626
• 关键词: Animals; Antibodies; Antigens; B-Lymphocytes; Baculoviruses; Biochemical Markers; Bioinformatics; Cancer Biology; Cancer Center; Cancer Patient; Cells; Clinical; Complex; Core Facility; Coupled; Custom; Development; Diagnosis; Disease Marker; Epitopes; Fluorescence; Future; Goals; Hybridomas; Immune response; Immunoassay; Individual; Liquid Chromatography; MHC Class I Genes; Malignant Neoplasms; Mass Spectrum Analysis; Modeling; Molecular Profiling; Molecular and Cellular Biology; Monitor; Multiprotein Complexes; Nature; Operative Surgical Procedures; Pathway interactions; Post Translational Modification Analysis; Post-Translational Protein Processing; Protein Analysis; Protein Chemistry; Proteins; Proteome; Proteomics; Purpose; RNA Processing; Range; Reagent; Recombinant Proteins; Research; Research Personnel; Resource Sharing; Resources; Schedule; Services; Signal Pathway; System; T-Lymphocyte; Techniques; Technology; Tissues; Two-Dimensional Gel Electrophoresis; base; cancer genomics; cooking; cost effective; genetic regulatory protein; instrumentation; interest; mecarzole; member; novel therapeutics; polypeptide; protein expression; response; therapeutic target; tool; tumor

The ability to simultaneously identify, quantitate, and analyze a large mixture of proteins between different functional states of cells and tissues is the ultimate goal of proteomics, and has the potential to identify signaling pathways and complex protein networks important in cancer biology. Because of the extremely diverse nature of protein expression through post-translational modifications (PTM) and RNA processing, and the wide dynamic range of expression, no single platform exists for quantitative differential analysis of global protein expression. This Shared Resource will therefore integrate three facilities and technological approaches to create a comprehensive proteomic effort by the BCM Cancer Center: 1) protein chemistry/ MS-based proteomics for identification and analysis (PTM-postranslational analysis) of individual proteins; 2) expression of recombinant proteins in the baculovirus system and as monoconal antibodies (MAbs) from B-cell hybridomas, and 3) isolation, identification, and MS-based analysis of large protein complexes or "interactomes". The Protein Chemistry/MS-Based Proteomics component, under the direction of Dr. Richard Cook, has existed as a core facility at Baylor since 1989. The Recombinant Protein Expression components were started as new services in September of 2005 with the recruitment of Dr. Dean Edwards to Baylor. The interactome, or Pathway Discovery, component will be started as a new service under the direction of Dr. Jun Qin, a recognized leader in immuno-isolation and identification of regulatory protein complexes by MS. In addtion to directing the Baculovirus/MAb facility, Dr. Edwards will serve as the overall Leader of the Shared Resource to integrate the scientiific and administrative interactions among these three components. The Protein Chemistry/MS-Based Proteomic facility is a successful and cost effective resource that provided services for 67 Baylor investigators in 2005, of which 40% were Cancer Center members. The Baculovirus/MAb facility has only been in operation since September of 2005 and has already provided services for 11 investigators and has six projects scheduled. Global differential protein expression profiling will be developed as a future service by combining separation of proteins by two-dimensional gel electrophoresis or liquid chromatography coupled with high-through put MS. Additionally, we plan to develop multiplex immunoassay systems based on Luminex flow cytometric fluorescence bead technology.

同时识别、定量和分析不同蛋白质之间的大量蛋白质混合物的能力 细胞和组织的功能状态是蛋白质组学的最终目标，并且有潜力识别 信号通路和复杂的蛋白质网络在癌症生物学中很重要。由于极其 通过翻译后修饰 (PTM) 和 RNA 加工实现蛋白质表达的多样性， 以及广泛的表达动态范围，不存在用于定量差异分析的单一平台 全局蛋白表达。 因此，该共享资源将整合三种设施和技术方法，以创建一个 BCM 癌症中心的全面蛋白质组学工作：1) 蛋白质化学/基于 MS 的蛋白质组学 单个蛋白质的鉴定和分析（PTM-翻译后分析）； 2）表达 杆状病毒系统中的重组蛋白以及来自 B 细胞的单克隆抗体 (MAb) 杂交瘤，以及 3) 大蛋白质复合物的分离、鉴定和基于 MS 的分析或 “相互作用组”。蛋白质化学/基于 MS 的蛋白质组学部分，在 Richard 博士的指导下 Cook 自 1989 年以来一直作为贝勒的核心设施而存在。重组蛋白表达组件 2005 年 9 月，随着 Dean Edwards 博士被贝勒聘用，该服务开始作为一项新服务。这 交互组（或 Pathway Discovery）组件将在 Dr. 的指导下作为一项新服务启动。 秦军，免疫分离和 MS 鉴定调节蛋白复合物领域公认的领导者。 除了指导杆状病毒/单克隆抗体设施外，Edwards 博士还将担任该设施的总体领导者。 共享资源以整合这三个组成部分之间的科学和管理交互。 基于蛋白质化学/MS 的蛋白质组学设施是一种成功且具有成本效益的资源，它提供了 2005 年为 67 名贝勒研究人员提供服务，其中 40% 是癌症中心成员。这 杆状病毒/单克隆抗体设施自 2005 年 9 月才开始运营，并已提供 为 11 名研究人员提供服务，并已安排 6 个项目。全局差异蛋白表达谱 将通过结合二维凝胶分离蛋白质来开发为未来的服务 电泳或液相色谱与高通量 MS 联用。此外，我们计划开发 基于 Luminex 流式细胞术荧光珠技术的多重免疫分析系统。