Animal Core

目前，囊性纤维化的唯一动物模型是小鼠。这些动物是很好的模型， CF的肠道疾病，鼻电位差模仿人类对应物，并且存在很大的 自发性人类肺部感染与小鼠中创建的感染模型之间的相似性， 使Cftr突变失效然而，这些老鼠很难繁殖，而且很脆弱。我们创建了 Animal Core以确保我们的研究者能够获得这一重要模型。动物的目标 核心是：1）为研究者提供良好表征的、遗传定义的、无特定病原体的小鼠 与CF相关的主题2)通过培训或直接履行各种职责来协助调查人员 操作，如建立感染的小鼠模型（通常是铜绿假单胞菌），给小鼠施用 药物，记录结果指标，如鼻电位差，支气管肺泡灌洗，或 定量肺形态测定3）管理与繁殖群体相关的记录，实验 程序或样品储存。这些服务是为凯斯西方的两名调查员提供的 储备大学和世界各地的其他CF研究人员。超过35名调查人员 在过去的五年里，病例接受了我们的特征良好的小鼠，以及16名研究人员， 案子在过去的五年里，CF感染小鼠模型的价值已经变得清晰，但也有 创造它们所需的陡峭的学习曲线。动物核心可以指示感兴趣的 这些模型中的研究者或为研究者进行研究。30多名外部调查人员 他们要么接受过核心组织的实验，要么接受过他们的培训， 使用这些服务。其中许多国家利用了美洲协调理事会议定书提供的援助。我们 我希望在下一个资助期内继续这种水平的活动，无论是在案件内外。 小鼠仅可通过一个来源商购，并且在那里它们被冷冻保存，并且必须 为了销售而培养的NIH支持的设施，如我们的设施，服务于社区的重要需求。

At present, the only animal models for cystic fibrosis are mice. These animals are excellent models for the intestinal disease of CF, the nasal potential difference mimics the human counterpart, and there are great similarities between the spontaneous human lung infection and the created infection models in the mice with disabling mutations in Cftr. However, these mice are difficult to breed and fragile. We have created the Animal Core to assure availability of this important model for our investigators. The goals of the Animal Core are: 1) to provide well-characterized, genetically defined, specific pathogen free mice to investigators for CF-related subjects. 2) to assist investigators either by training or by direct performance in various manipulations, such as creating mouse models of infection (usually with P. aeruginosa), dosing mice with drugs, recording outcome measures such as nasal potential difference, bronchoalveolar lavage, or quantitative lung morphometry 3) to manage records associated with breeding colonies, experimental procedures, or sample storage. These services are provided for both investigators at Case Western Reserve University and other CF investigators around the world. More than 35 investigators outside of Case have received our well-characterized mice during the last five years, as well as 16 investigators at Case. Over the last five years, the value of mouse models of infection in CF has become clear, but so has the steep learning curve required to create them. The Animal Core can either instruct interested investigators in these models or perform the studies for investigators. More than 30 outside investigators either had experiments done by the Core or received training from them, and 13 investigators at Case accessed these services. Many of them took advantage of the assistance with IACUC protocols. We expect to continue this level of activity in the next grant period, both within Case and outside of it. Since CF mice are available commercially only through one source, and there they are cryopreserved and must be brought up for sale, NIH-supported facilities such as ours serve an important need in the community.