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Animal Core

动物核心

基本信息

批准号：
7288640
负责人：
Mitchell L Drumm
金额：
$ 19.13万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7288640
关键词：
Alleles Anesthesia procedures Animal Model Animals Applications Grants Area Bacterial Counts Body Weight Breeding Bronchoalveolar Lavage Bronchoalveolar Lavage Fluid Caring Cell Count Characteristics Clinical Communities Count Cryopreservation Cystic Fibrosis Data Databases Development Disease Doctor of Philosophy Dose Eicosanoids Electronic Mail Equipment Euthanasia Flow Cytometry Funding Genes Genetic Genotype Goals Grant Hand Harvest Histology Human IACUC Infection Inhalant dose form Injection of therapeutic agent Institution Insufflation International Internet Intestinal Diseases Intraperitoneal Injections Learning Liquid substance Lung Mails Measurement Messenger RNA Microscope Modeling Mouse Strains Mus Mutation Nose Numbers Operative Surgical Procedures Outcome Measure Oxidative Stress Pathway Paraffin Performance Pharmaceutical Preparations Phenotype Polymerase Chain Reaction Procedures Process Protocols documentation Pseudomonas aeruginosa Publications Records Relative (related person)Research Research Personnel Reverse Transcriptase Polymerase Chain Reaction Sales Sampling Sepharose Serum Services Site Source Specific Pathogen Frees Specific qualifier value Subcutaneous Injections System Telephone Testing Tissues Tracheostomy procedure Training Transgenes Universities Urea Vaporizer animal resource beneficiary c new cystic fibrosis mouse cytokine data management genetic pedigree germ free condition interest intravenous injection morphometry mouse model mucoid mutant pre-clinical research study therapy development

项目摘要

At present, the only animal models for cystic fibrosis are mice. These animals are excellent models for the intestinal disease of CF, the nasal potential difference mimics the human counterpart, and there are great similarities between the spontaneous human lung infection and the created infection models in the mice with disabling mutations in Cftr. However, these mice are difficult to breed and fragile. We have created the Animal Core to assure availability of this important model for our investigators. The goals of the Animal Core are: 1) to provide well-characterized, genetically defined, specific pathogen free mice to investigators for CF-related subjects. 2) to assist investigators either by training or by direct performance in various manipulations, such as creating mouse models of infection (usually with P. aeruginosa), dosing mice with drugs, recording outcome measures such as nasal potential difference, bronchoalveolar lavage, or quantitative lung morphometry 3) to manage records associated with breeding colonies, experimental procedures, or sample storage. These services are provided for both investigators at Case Western Reserve University and other CF investigators around the world. More than 35 investigators outside of Case have received our well-characterized mice during the last five years, as well as 16 investigators at Case. Over the last five years, the value of mouse models of infection in CF has become clear, but so has the steep learning curve required to create them. The Animal Core can either instruct interested investigators in these models or perform the studies for investigators. More than 30 outside investigators either had experiments done by the Core or received training from them, and 13 investigators at Case accessed these services. Many of them took advantage of the assistance with IACUC protocols. We expect to continue this level of activity in the next grant period, both within Case and outside of it. Since CF mice are available commercially only through one source, and there they are cryopreserved and must be brought up for sale, NIH-supported facilities such as ours serve an important need in the community.

目前，囊性纤维化的唯一动物模型是小鼠。这些动物是优秀的模型 CF肠道疾病，鼻电位差模仿人类对应物，并且有很大人类自发肺部感染与小鼠肺部感染模型之间的相似之处禁用 Cftr 中的突变。然而，这些小鼠难以繁殖且脆弱。我们已经创建了 Animal Core 确保我们的研究人员可以使用这一重要模型。动物的目标核心是：1）为研究人员提供特征明确、基因明确、无特定病原体的小鼠适用于 CF 相关科目。 2) 通过培训或直接执行各种任务来协助调查人员操作，例如创建感染小鼠模型（通常是铜绿假单胞菌）、给小鼠注射药物，记录结果测量，例如鼻电位差、支气管肺泡灌洗或定量肺形态测量 3) 管理与繁殖群体、实验相关的记录程序或样品存储。这些服务是为 Case Western 的两名调查员提供的储备大学和世界各地的其他 CF 研究人员。超过 35 名调查员 Case 在过去五年中接收了我们特征明确的小鼠，以及 16 名研究人员案件。在过去的五年里，CF 感染小鼠模型的价值已经变得显而易见，但也同样如此。创建它们所需的陡峭学习曲线。动物核心可以指导感兴趣的研究人员在这些模型中或为研究人员进行研究。 30多名外部调查员 Case 的 13 名调查员要么接受过 Core 的实验，要么接受过 Core 的培训访问了这些服务。他们中的许多人利用了 IACUC 协议的帮助。我们期望在下一个资助期内继续这种水平的活动，无论是在 Case 内部还是外部。自从CF 小鼠只能通过一种来源在商业上获得，并且它们被冷冻保存并且必须像我们这样的 NIH 支持的设施被出售，以满足社区的重要需求。