Animal Core

动物核心

• 批准号: 7288640
• 负责人: Mitchell L Drumm
• 金额: $ 19.13万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7288640
• 关键词: Alleles; Anesthesia procedures; Animal Model; Animals; Applications Grants; Area; Bacterial Counts; Body Weight; Breeding; Bronchoalveolar Lavage; Bronchoalveolar Lavage Fluid; Caring; Cell Count; Characteristics; Clinical; Communities; Count; Cryopreservation; Cystic Fibrosis; Data; Databases; Development; Disease; Doctor of Philosophy; Dose; Eicosanoids; Electronic Mail; Equipment; Euthanasia; Flow Cytometry; Funding; Genes; Genetic; Genotype; Goals; Grant; Hand; Harvest; Histology; Human; IACUC; Infection; Inhalant dose form; Injection of therapeutic agent; Institution; Insufflation; International; Internet; Intestinal Diseases; Intraperitoneal Injections; Learning; Liquid substance; Lung; Mails; Measurement; Messenger RNA; Microscope; Modeling; Mouse Strains; Mus; Mutation; Nose; Numbers; Operative Surgical Procedures; Outcome Measure; Oxidative Stress Pathway; Paraffin; Performance; Pharmaceutical Preparations; Phenotype; Polymerase Chain Reaction; Procedures; Process; Protocols documentation; Pseudomonas aeruginosa; Publications; Records; Relative (related person); Research; Research Personnel; Reverse Transcriptase Polymerase Chain Reaction; Sales; Sampling; Sepharose; Serum; Services; Site; Source; Specific Pathogen Frees; Specific qualifier value; Subcutaneous Injections; System; Telephone; Testing; Tissues; Tracheostomy procedure; Training; Transgenes; Universities; Urea; Vaporizer; animal resource; beneficiary; c new; cystic fibrosis mouse; cytokine; data management; genetic pedigree; germ free condition; interest; intravenous injection; morphometry; mouse model; mucoid; mutant; pre-clinical; research study; therapy development

At present, the only animal models for cystic fibrosis are mice. These animals are excellent models for the intestinal disease of CF, the nasal potential difference mimics the human counterpart, and there are great similarities between the spontaneous human lung infection and the created infection models in the mice with disabling mutations in Cftr. However, these mice are difficult to breed and fragile. We have created the Animal Core to assure availability of this important model for our investigators. The goals of the Animal Core are: 1) to provide well-characterized, genetically defined, specific pathogen free mice to investigators for CF-related subjects. 2) to assist investigators either by training or by direct performance in various manipulations, such as creating mouse models of infection (usually with P. aeruginosa), dosing mice with drugs, recording outcome measures such as nasal potential difference, bronchoalveolar lavage, or quantitative lung morphometry 3) to manage records associated with breeding colonies, experimental procedures, or sample storage. These services are provided for both investigators at Case Western Reserve University and other CF investigators around the world. More than 35 investigators outside of Case have received our well-characterized mice during the last five years, as well as 16 investigators at Case. Over the last five years, the value of mouse models of infection in CF has become clear, but so has the steep learning curve required to create them. The Animal Core can either instruct interested investigators in these models or perform the studies for investigators. More than 30 outside investigators either had experiments done by the Core or received training from them, and 13 investigators at Case accessed these services. Many of them took advantage of the assistance with IACUC protocols. We expect to continue this level of activity in the next grant period, both within Case and outside of it. Since CF mice are available commercially only through one source, and there they are cryopreserved and must be brought up for sale, NIH-supported facilities such as ours serve an important need in the community.

目前，囊性纤维化的动物模型只有小鼠。这些动物是 肠道疾病的CF，鼻电位的差异模仿人类的对应物，而且有很大的 自发性人肺感染与所建立的小鼠感染模型的相似性 使cftr基因突变失效。然而，这些小鼠很难饲养，而且很脆弱。我们已经创建了 动物核心，以确保这一重要模型的可用性为我们的研究人员。《动物的目标》 核心是：1)向研究人员提供具有良好特征、基因定义良好、无特定病原体的小鼠 对于与CF相关的科目。2)通过培训或直接在各种不同方面的表现来协助调查人员 操作，如建立小鼠感染模型(通常是铜绿假单胞菌感染)，给小鼠注射 药物，记录结果测量，如鼻电位差，支气管肺泡灌洗，或 定量肺形态测量法3)管理与繁殖群体相关的记录，实验性 程序，或样品存储。这些服务是为凯斯韦斯特的两名调查人员提供的 储备大学和世界各地的其他CF调查人员。超过35名调查人员在国外 在过去的五年里，Case已经收到了我们特征良好的老鼠，以及16名调查人员 凯斯。在过去的五年里，慢性萎缩性胃炎的小鼠感染模型的价值已经变得显而易见，但也是如此 创建它们所需的陡峭的学习曲线。动物核心可以指示感兴趣的人 调查人员在这些模型中或为调查人员执行研究。30多名外部调查人员 要么由核心进行了实验，要么接受了他们的培训，以及Case的13名调查人员 访问了这些服务。他们中的许多人利用了IACUC协议的协助。我们 预计在下一个赠款期间，无论是在案件内部还是在案件之外，都将继续开展这种水平的活动。自CF以来 小鼠只能通过一个来源获得，在那里它们被冷冻保存，并且必须 在出售过程中，像我们这样由NIH支持的设施满足了社区的重要需求。