Mouse Models

鼠标型号

• 批准号: 8705743
• 负责人: Mitchell L Drumm
• 金额: $ 8.24万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8705743
• 关键词: Alleles; Animal Model; Animals; Australia; Breeding; Canada; Caring; Characteristics; Communities; Comparative Study; Complement; Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Data; Data Collection; Database Management Systems; Databases; Disease; Dose; Environment; Europe; Family suidae; Ferrets; Functional disorder; Generations; Genetic; Goals; Grant; Human; Infection; International; Laboratories; Length; Lung; Mammalian Genetics; Mammals; Modeling; Mouse Strains; Mus; Nature; Pharmaceutical Preparations; Phenotype; Physiology; Procedures; Publications; Recording of previous events; Records; Research; Research Personnel; Resources; Services; Source; System; Testing; Time; Tissues; Training; Transgenic Organisms; Translational Research; Weight; Work; animal colony; cell type; computer program; congenic; cost; cystic fibrosis mouse; in vivo; in vivo Model; mouse model; mutant; programs; research study; restoration; therapy development; tool

The mouse has been a critical component of our program's ability to examine CF questions in an in vivo setting. We have compiled a large list of CF or CF-related mouse models (over 50) and have made them congenic to provide isogentic tools to the research community. This resource has become an international resource, with nearly 60 investigators using the Core (16 local and 38 across the US, Canada, Europe and Australia). We have set up MTAs that are as non-restrictive as we can make them so that CF research is not impeded. We have developed CF models ourselves, most recently conditional (floxed) alleles of Cftr and are currently generating mice carrying human CFTR to allow additional questions to be answered. With the advent of the CF pig and ferret, the mouse models we use also provide the opportunity for comparative studies. As is apparent with all CF animal models to date, the CF mouse is fragile and requires special husbandry, a service most effectively provided by a core. The overall goal of the CF Mouse Models Core is to provide mouse models of CF for understanding the pathophysiology of the disease and for development of therapies. To continue to accomplish our goals, the specific aims of the CF Mouse Models Core are: To provide well-characterized CF mouse models to the institutional, regional, national and international CF research community. Expedite data collection for preliminary studies and facilitate training in experimental procedures involving CF mouse models. We provide many services to CF investigators that include collecting phenotypic data (e.g., weight, length, survival etc.,) and obtaining tissues from CF mouse strains, carrying out lung infection and/or drug dosing experiments as well as training in CF mouse handling and care. These services are utilized daily by institutional users and frequently by external users. To maintain a centralized database that contains breeding history, phenotypes observed and experimental records of the various CF mouse strains in the Core.

小鼠一直是我们的程序的能力，在体内环境中检查CF问题的关键组成部分。我们已经编制了大量CF或CF相关小鼠模型（超过50种），并使其同源，为研究界提供同源工具。该资源已成为一个国际资源，近60名研究人员使用核心（16名当地研究人员和38名美国、加拿大、欧洲和澳大利亚的研究人员）。我们已经建立了尽可能非限制性的MTA，以便CF研究不受阻碍。我们自己开发了CF模型，最近是Cftr的条件（floxed）等位基因，目前正在产生携带人类CFTR的小鼠，以回答更多的问题。随着CF猪和雪貂的出现，我们使用的小鼠模型也为比较研究提供了机会。正如迄今为止所有CF动物模型所显示的，CF小鼠是脆弱的，需要特殊的饲养，这是由核心提供的最有效的服务。CF小鼠模型核心的总体目标是提供CF小鼠模型，用于了解疾病的病理生理学和开发治疗方法。 为了继续实现我们的目标，CF小鼠模型核心的具体目标是： 为机构、区域、国家和国际CF研究界提供良好表征的CF小鼠模型。 加快初步研究的数据收集，并促进涉及CF小鼠模型的实验程序的培训。我们为CF研究者提供许多服务，包括收集表型数据（例如，重量、长度、存活率等，）以及从CF小鼠品系获得组织，进行肺部感染和/或药物给药实验以及CF小鼠处理和护理方面的训练。机构用户每天都会使用这些服务，外部用户也经常使用这些服务。 维护一个集中数据库，其中包含核心中各种CF小鼠品系的育种历史、观察到的表型和实验记录。