Mouse Models

鼠标型号

• 批准号: 8705743
• 负责人: Mitchell L Drumm
• 金额: $ 8.24万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8705743
• 关键词: Alleles; Animal Model; Animals; Australia; Breeding; Canada; Caring; Characteristics; Communities; Comparative Study; Complement; Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Data; Data Collection; Database Management Systems; Databases; Disease; Dose; Environment; Europe; Family suidae; Ferrets; Functional disorder; Generations; Genetic; Goals; Grant; Human; Infection; International; Laboratories; Length; Lung; Mammalian Genetics; Mammals; Modeling; Mouse Strains; Mus; Nature; Pharmaceutical Preparations; Phenotype; Physiology; Procedures; Publications; Recording of previous events; Records; Research; Research Personnel; Resources; Services; Source; System; Testing; Time; Tissues; Training; Transgenic Organisms; Translational Research; Weight; Work; animal colony; cell type; computer program; congenic; cost; cystic fibrosis mouse; in vivo; in vivo Model; mouse model; mutant; programs; research study; restoration; therapy development; tool

The mouse has been a critical component of our program's ability to examine CF questions in an in vivo setting. We have compiled a large list of CF or CF-related mouse models (over 50) and have made them congenic to provide isogentic tools to the research community. This resource has become an international resource, with nearly 60 investigators using the Core (16 local and 38 across the US, Canada, Europe and Australia). We have set up MTAs that are as non-restrictive as we can make them so that CF research is not impeded. We have developed CF models ourselves, most recently conditional (floxed) alleles of Cftr and are currently generating mice carrying human CFTR to allow additional questions to be answered. With the advent of the CF pig and ferret, the mouse models we use also provide the opportunity for comparative studies. As is apparent with all CF animal models to date, the CF mouse is fragile and requires special husbandry, a service most effectively provided by a core. The overall goal of the CF Mouse Models Core is to provide mouse models of CF for understanding the pathophysiology of the disease and for development of therapies. To continue to accomplish our goals, the specific aims of the CF Mouse Models Core are: To provide well-characterized CF mouse models to the institutional, regional, national and international CF research community. Expedite data collection for preliminary studies and facilitate training in experimental procedures involving CF mouse models. We provide many services to CF investigators that include collecting phenotypic data (e.g., weight, length, survival etc.,) and obtaining tissues from CF mouse strains, carrying out lung infection and/or drug dosing experiments as well as training in CF mouse handling and care. These services are utilized daily by institutional users and frequently by external users. To maintain a centralized database that contains breeding history, phenotypes observed and experimental records of the various CF mouse strains in the Core.

小鼠是我们的程序在体内环境中检查 CF 问题的能力的关键组成部分。我们编制了大量 CF 或 CF 相关小鼠模型（超过 50 个），并使它们成为同类模型，为研究界提供同源工具。该资源已成为国际资源，有近 60 名研究人员使用该核心（16 名本地研究人员，38 名来自美国、加拿大、欧洲和澳大利亚）。我们已经建立了尽可能非限制性的 MTA，以便 CF 研究不会受到阻碍。我们自己开发了 CF 模型，最近是 Cftr 的条件（floxed）等位基因，目前正在生成携带人类 CFTR 的小鼠，以回答其他问题。随着CF猪和雪貂的出现，我们使用的小鼠模型也提供了进行比较研究的机会。迄今为止，所有 CF 动物模型都明显看出，CF 小鼠很脆弱，需要特殊饲养，而核心提供的服务最有效。 CF 小鼠模型核心的总体目标是提供 CF 小鼠模型，以了解该疾病的病理生理学并开发治疗方法。 为了继续实现我们的目标，CF 鼠标模型核心的具体目标是： 为机构、区域、国家和国际 CF 研究界提供特征良好的 CF 小鼠模型。 加快初步研究的数据收集，并促进涉及 CF 小鼠模型的实验程序的培训。我们为 CF 研究人员提供许多服务，包括收集表型数据（例如体重、身长、存活率等）并从 CF 小鼠品系中获取组织、进行肺部感染和/或药物剂量实验以及 CF 小鼠处理和护理培训。这些服务每天被机构用户使用，并且经常被外部用户使用。 维护一个集中数据库，其中包含核心中各种 CF 小鼠品系的繁殖历史、观察到的表型和实验记录。