MOUSE PHENOTYPING, PHYSIOLOGY, AND METABOLISM CORE

小鼠表型、生理学和代谢核心

• 批准号: 7284633
• 负责人: REXFORD S. AHIMA
• 金额: $ 17.3万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7284633
• 关键词: Arts; Biological Markers; Body Composition; Bone Tissue; Cellular biology; Chemicals; Chemistry; Consult; Consultations; Coupled; Data; Data Collection; Databases; Diabetes Mellitus; Diabetic mouse; Diagnostic; Diagnostic Services; Diagnostic tests; Diet; Dose; Employment; Endocrinology; Energy Metabolism; Ensure; Environmental Risk Factor; Equipment; Exercise; Experimental Designs; Failure; Fatty acid glycerol esters; Fee-for-Service Plans; Funding; Funding Agency; Future; Gene Targeting; Genomics; Glucose; Home environment; Hormonal; Housing; Human; Human Resources; Injection of therapeutic agent; Insulin; Insulin Resistance; Intravenous; Islet Cell; Islets of Langerhans; Kinetics; Knockout Mice; Laboratories; Laboratory Animals; Laboratory mice; Lipids; Magnetic Resonance; Magnetic Resonance Imaging; Measurement; Measures; Metabolic; Metabolism; Methodology; Mission; Molecular; Monitor; Motor Activity; Mus; Muscle; Mutant Strains Mice; Neonatal; Obese Mice; Obesity; Operative Surgical Procedures; Oral; Oxygen Consumption; Pathogenesis; Pharmacological Treatment; Phenotype; Physiological; Physiology; Procedures; Radioactive Tracers; Radioimmunoassay; Research; Research Personnel; Rest; Screening procedure; Services; Specialist; System; Temperature; Testing; Tissues; Transgenic Organisms; Translations; United States National Institutes of Health; Water; Week; Wolves; blood glucose regulation; cost; data acquisition; day; design; drinking; feeding; glucose uptake; in vivo; instrument; insulin sensitivity; insulin tolerance; intraperitoneal; member; mouse model; research study; respiratory

MOUSE PHENOTYPING, PHYSIOLOGY, AND METABOLISM CORE: Director - R. Ahima Our understanding of the pathogenesis of diabetes has benefited from the use of gene targeting methodology in mice to elucidate molecular mechanisms. However, such efforts are often hampered by an absence of a clear metabolic phenotype. Failure to identify a phenotype may be due to lack of expertise and/or facilities for evaluating metabolic changes in mice. The Mouse Phenotyping, Physiology and Metabolism Core provides investigators of the Penn Diabetes and Endocrinology Research Center (DERC) with state-of-the-art, timely and cost-effective diagnostic studies in mice. The core offers consultation and experimental design, monitoring of feeding, energy expenditure and locomotor activity using the Comprehensive Laboratory Animal Monitoring System (CLAMS), treadmill exercise using the Oxymax system, and measurement of body composition using dual emission x-ray absorptiometry (DEXA) and carcass chemistry. Glucose homeostasis is assessed by oral or intraperitoneal (i.p.) glucose administration, and whole body insulin sensitivity by i.p. insulin injection. Insulin clamp and radioactive tracers are used to assess glucose fluxes and tissue specific glucose uptake. Studies in the core are performed by two research specialists under the direction of Rex Ahima. Future plans for the core include the use magnetic resonance (MRI) for measurement of water, lean and fat content, assessment of in vivo lipid kinetics, and employment of an additional technician to expedite services. The Mouse Phenotyping, Physiology and Metabolism Core will maintain a databank of metabolic and hormonal parameters in mouse models of diabetes and obesity, and coordinate its activities with other core laboratories, i.e. Islet Cell Biology (Franz Matschinsky), Radioimmunoassay/Biomarkers (Bryan Wolf; Muredach Reilly), Transgenic and Chimeric Mouse (Nancy Cooke), and Genomics and Gene Targeting Cores (Klaus Kaestner). These efforts will result in optimum data acquisition and metabolic phenotyping of mice, and facilitate the translation of ideas from the bench to mice, and ultimately to humans.

小鼠表型、生理学和代谢核心：负责人- R。AHIMA 我们对糖尿病发病机制的理解得益于基因靶向的使用 方法在小鼠中阐明分子机制。然而，这种努力往往 由于缺乏明确的代谢表型而受阻。无法识别表型可能是 这是由于缺乏评估小鼠代谢变化的专业知识和/或设施。鼠标 表型，生理学和代谢核心提供了宾夕法尼亚州糖尿病和糖尿病的研究人员， 内分泌学研究中心（DERC）提供最先进、及时和具有成本效益的诊断 在老鼠身上的研究核心提供咨询和实验设计，喂养监测， 能量消耗和运动活动，使用综合实验室动物监测 系统（CLAMS），使用Oxymax系统的跑步机锻炼，以及身体测量 使用双发射X射线吸收测定法（DEXA）和屠体化学测定组合物。葡萄糖 通过口服或腹膜内（i. p.）葡萄糖给药和全身 通过腹膜内胰岛素注射测定胰岛素敏感性。胰岛素钳夹和放射性示踪剂用于评估 葡萄糖通量和组织特异性葡萄糖摄取。核心研究由两名 在雷克斯·阿希玛的指导下，未来的核心计划包括使用 磁共振（MRI）用于测量水、瘦肉和脂肪含量，评估体内 脂质动力学，并雇用额外的技术人员，以加快服务。鼠标 表型、生理学和代谢核心将维护一个代谢和激素的数据库， 参数在小鼠糖尿病和肥胖模型，并协调其活动与其他核心 实验室，即胰岛细胞生物学（Franz Matschinsky）、放射免疫测定/生物标志物（Bryan Wolf; Muredach Reilly）、转基因和嵌合小鼠（Nancy Cooke）以及基因组学和基因 瞄准核心（克劳斯·凯斯特纳）。这些努力将导致最佳的数据采集， 小鼠的代谢表型，并促进从实验室到小鼠的想法的翻译， 最终是人类。