CORE--TRANSGENIC/KNOCKOUT ANIMAL FACILITY

核心——转基因/基因敲除动物设施

• 批准号: 7406610
• 负责人: STEPHEN JONES
• 金额: $ 9.82万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7406610
• 关键词: Alleles; Animal Model; Biochemical; Biomedical Research; Communities; Cryopreservation; DNA; Disease; Embryo; Gene Targeting; Genes; Genetic; Information Resources; Injection of therapeutic agent; Knock-out; Microinjections; Mission; Modeling; Molecular Biology; Mus; Mutation; Process; Protocols documentation; Rattus; Research Personnel; Rodent; Rodent Model; Role; Scientist; Transgenic Animals; Transgenic Mice; Transgenic Mouse Facility; Transgenic Organisms; Work; animal facility; blastocyst; cost; embryonic stem cell; knockout animal; medical schools; member; mouse model; research study; size

Mouse genetics has proven to be extremely valuable to biomedical research by permiting the investigator to examine the role of genes involved in a wide variety of disease processes and in validating or correcting the results of biochemical and molecular biology experiments. The ability to generate specific mutations in genes as well as conditional alleles of genes has greatly expanded the repetoire of transgenic and knockout model s available for study. To meet this ever-increasing demand for mouse models, DM ASS Medical School has an established Transgenic Animal Modeling Core. Since its inception in 1991, the mission of the Transgenic Animal Modeling Core is to produce genetically-altered mice for the UMASS Medical School community in a timely and cost-efficient manner. The size and scope of the Core was greatly expanded in 1997 and is now composed of two facilities, the Gene Targeting Facility- performing gene targeted ES cell work, and the Transgenic Mouse Facility, performing pronuclear injections, blastocyst injections, cryopreservation, and rederivation. In the last five years the Core has generated more than 250 different lines of transgenic mice, targeted over 40 genes in mouse ES cells, produced over 50 lines of gene-targeted mice via blastocyst injection, and has cryopreserved more than 35 lines of mice for UMASS researchers. In addition, the Core acts as a resource for information, protocols, and assistance for UMASS investigators working with genetically altered rodents and for those scientists performing ES cell work in their own labs. Furthermore, the Core now offers microinjection of DNA and lentiviral constructs into rat embryos in order to generate transgenic rats. Greater than 70% of all rodent models generated by the Transgenic Animal Modeling Core have been for UMASS DERC members.

事实证明，小鼠遗传学对生物医学研究非常有价值 研究者研究了涉及多种疾病过程的基因的作用，并验证或纠正生化和分子生物学实验的结果。在基因以及基因的条件等位基因中产生特定突变的能力大大扩展了可用于研究的转基因和基因敲除模型的重复。为了满足这种对小鼠模型的不断增长的需求，DM ASS医学院拥有已建立的转基因动物建模核心。自1991年成立以来，转基因动物建模核心的使命是及时且经济高效的方式为UMass医学院社区生产遗传变化的小鼠。核心的大小和范围在1997年大大扩展，现在由两个设施组成，即靶向设施的基因 基因针对ES的细胞工作，而转基因小鼠的设施进行了前核注射，胚泡注射，冷冻保存和重新启动。在过去的五年中，核心已经产生了250多种不同的转基因小鼠，这些小鼠针对了小鼠ES细胞中的40多个基因，通过胚泡注射产生了50种基因靶向的小鼠，并为UMASS研究人员提供了35种以上的小鼠。此外，核心还可以作为对啮齿动物的转基因研究人员的信息，协议和帮助的资源，以及那些在自己的实验室中执行ES细胞工作的科学家。此外，现在的核心将DNA和慢病毒构建体的显微注射提供到大鼠胚胎中，以产生转基因大鼠。转基因动物建模核心产生的所有啮齿动物模型中，超过70％是针对UMass DERC成员的。