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Identification of Novel Components of the Dystrophin Complex Using C. Elegans

使用线虫鉴定肌营养不良蛋白复合物的新成分

基本信息

批准号：
7501975
负责人：
Hongkyun Kim
金额：
$ 4.11万
依托单位：
ERNEST GALLO CLINIC AND RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-30 至 2008-08-01
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7501975
关键词：
Acetylcholine Animal Model Biochemical Caenorhabditis elegans Candidate Disease Gene Cell membrane Cell physiology Cellular biology Chromosome Mapping Class Complement Complex Condition Decompression Sickness Defect Development Diagnosis Disease Dystrophin Dystrophin-Associated Protein Complex Exhibits Genes Genetic Genetic Screening Head Homologous Gene Human In Vitro Lead Mammals Maps Mediating Mediator of activation protein Molecular Molecular Genetics Mus Muscle Muscular Dystrophies Mutation Nematoda Pathogenesis Pathway interactions Patients Pattern Phenotype Physiological Play Proteins Research Role Severities Signal Transduction Skeletal Muscle System Techniques Therapeutic Tissue-Specific Gene Expression Tissues Transport Process acetylcholine transporter base choline transporter design gene cloning improved in vivo mutant novel novel therapeutics trafficking

项目摘要

DESCRIPTION (provided by applicant): The dystrophin associated protein complex is a multimeric protein complex found in many different tissues, including muscle. Genetic defects in the dystrophin associated protein complex lead to muscular dystrophy in humans. The dystrophin associated protein complex plays several different roles in the plasma membrane. However, given that large percentages of patients with muscular dystrophy remain to be molecularly diagnosed, there is a possibility that some of the components may not have been identified yet. Furthermore, we do not know how the dystrophin complex is exactly assembled, processed and transported to the plasma membrane. The nematode C. elegans is an established genetic model organism, and possesses most of components of the dystrophin associated protein complex. In C. elegans, mutations in components of the dystrophin associated protein complex cause a unique locomotory phenotype that is not observed in any other class of uncoordinated or hyperactive mutants, and lead to muscle degeneration under certain conditions. We previously designed a genetic screen that identifies specifically mutants exhibiting the same locomotory phenotype as the dystrophin mutant, and identified several genes encoding known components of the dystrophin complex. Additionally, we identified a novel gene that encodes an acetylcholine/choline transporter. In a modified genetic screen we now have identified at least two additional novel genes. We have cloned one of the genes and are continuing to characterize the gene. We propose to expand the genetic screen to completion and identify mutants that exhibit the same locomotory phenotype as the dystrophin mutant. We will determine whether these mutants represent known genes or novel genes of the dystrophin associated complex. We will clone the novel genes by a combination of genetic mapping and transformation rescue. These novel genes may be unidentified components of the dystrophin complex, regulate assembly or trafficking of the complex, and mediate cellular functions. We will characterize the molecular functions of these novel genes using genetic, molecular and cell biology techniques. We will also identify and study the functional mammalian homologues. These findings will improve our understanding of the pathogenesis mechanism of muscular dystrophy in humans and may help to devise new therapeutic strategies.

描述（由申请人提供）：抗肌萎缩蛋白相关蛋白复合物是在许多不同组织（包括肌肉）中发现的多聚体蛋白复合物。抗肌萎缩蛋白相关蛋白复合物的遗传缺陷导致人类肌肉萎缩症。抗肌萎缩蛋白相关蛋白复合物在质膜中起几种不同的作用。然而，考虑到大部分肌营养不良症患者仍有待分子诊断，因此有可能尚未确定某些成分。此外，我们不知道肌营养不良蛋白复合物是如何准确组装、加工和运输到质膜的。线虫C.秀丽线虫是一种已建立的遗传模式生物，拥有肌营养不良蛋白相关蛋白复合物的大部分成分。In C.在线虫中，肌营养不良蛋白相关蛋白复合物组分的突变引起独特的运动表型，这在任何其他类别的不协调或过度活跃的突变体中未观察到，并在某些条件下导致肌肉变性。我们以前设计了一个遗传筛选，特别是识别突变体表现出相同的运动表型的肌营养不良蛋白突变体，并确定了几个基因编码的肌营养不良蛋白复合物的已知组件。此外，我们确定了一个新的基因，编码乙酰胆碱/胆碱转运蛋白。在改良的遗传筛选中，我们现在已经确定了至少两个额外的新基因。我们已经克隆了其中一个基因，并正在继续表征该基因。我们建议扩大遗传筛选完成，并确定突变体表现出相同的运动表型的抗肌萎缩蛋白突变体。我们将确定这些突变体是否代表已知的基因或新的基因的肌营养不良蛋白相关的复合物。我们将通过遗传作图和转化拯救相结合的方法克隆新基因。这些新基因可能是肌营养不良蛋白复合物的未鉴定组分，调节复合物的组装或运输，并介导细胞功能。我们将使用遗传学、分子生物学和细胞生物学技术来表征这些新基因的分子功能。我们还将鉴定和研究功能性哺乳动物同源物。这些发现将提高我们对人类肌营养不良症发病机制的了解，并可能有助于设计新的治疗策略。