Dissection of protective responses to heterologous Campylobacter vaccines

空肠弯曲杆菌是细菌性食源性人畜共患病的最大致病原因。家禽被弯曲杆菌繁殖到很高水平，与人类疾病之间有很强的联系。通过接种疫苗减少鸡体内弯曲杆菌的能力将极大地减少人类感染。最近有研究表明，沙门氏菌疫苗向鸡的免疫系统提供一种特定的弯曲杆菌蛋白(CjaA)，可以防止弯曲杆菌的定植。CjaA是氨基酸的转运体，我们已经确定了其他几个类似的转运体作为疫苗的候选者。我们已经证明，当弯曲杆菌在鸡的肠道中时，这些都是表达的。我们将在沙门氏菌表达这些其他运输蛋白的地方制造疫苗，并测试它们的保护作用。CjaA疫苗有效的原因尚不清楚。该沙门氏菌株仍具有一定的毒力特征，可能推动免疫的发展。我们将调查不同毒力的沙门氏菌疫苗株在针对CjaA的免疫反应发展中的作用。了解沙门氏菌毒力特性在疫苗功能中的作用将有助于开发更好的策略来控制弯曲杆菌。与BB/D000866/1连接。

Campylobacter jejuni is the largest cause of bacterial food borne zoonoses. There is a strong link between poultry, which are colonised to a high level by Campylobacter, and human disease. The ability to reduce Campylobacter in chickens by vaccination would greatly reduce human infection. Recently it has been shown that a Salmonella vaccine which presents a specific Campylobacter protein (CjaA), to the chicken immune system can protect against Campylobacter colonisation. CjaA is a transporter of amino-acids and we have identified several other similar transporters as candidates for vaccines. We have shown these are expressed when Campylobacter is in the intestines of the chicken. We will create vaccines where Salmonella expresses these other transport proteins and test them for protection. The reason why the CjaA vaccine works is not clear. The Salmonella strain that is used to present CjaA to the chicken vaccine still possesses some virulence traits which may drive the development of immunity. We will investigate the role of Salmonella vaccine strains which vary in their virulence, in the development of the immune response against CjaA. The understanding of the role of the Salmonella virulence properties in function of the vaccine will allow the development of improved strategies for control of Campylobacter. Joint with BB/D000866/1.