Dissection of protective responses to heterologous Campylobacter vaccines

异源弯曲杆菌疫苗保护性反应的剖析

• 批准号: BB/D00019X/1
• 负责人: Duncan Maskell
• 金额: $ 27.48万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD00019X%2F1
• 关键词: Dissection; protective; responses; heterologous; Campylobacter

Campylobacter jejuni is the largest cause of bacterial food borne zoonoses. There is a strong link between poultry, which are colonised to a high level by Campylobacter, and human disease. The ability to reduce Campylobacter in chickens by vaccination would greatly reduce human infection. Recently it has been shown that a Salmonella vaccine which presents a specific Campylobacter protein (CjaA), to the chicken immune system can protect against Campylobacter colonisation. CjaA is a transporter of amino-acids and we have identified several other similar transporters as candidates for vaccines. We have shown these are expressed when Campylobacter is in the intestines of the chicken. We will create vaccines where Salmonella expresses these other transport proteins and test them for protection. The reason why the CjaA vaccine works is not clear. The Salmonella strain that is used to present CjaA to the chicken vaccine still possesses some virulence traits which may drive the development of immunity. We will investigate the role of Salmonella vaccine strains which vary in their virulence, in the development of the immune response against CjaA. The understanding of the role of the Salmonella virulence properties in function of the vaccine will allow the development of improved strategies for control of Campylobacter. Joint with BB/D000866/1.

空肠弯曲菌是细菌性食源性人畜共患病的最大病原。家禽与人类疾病之间有着密切的联系，家禽被弯曲杆菌定殖到很高的水平。通过接种疫苗减少鸡中弯曲杆菌的能力将大大减少人类感染。最近已经显示，向鸡免疫系统呈递特异性弯曲杆菌蛋白（CjaA）的沙门氏菌疫苗可以保护免受弯曲杆菌定殖。CjaA是氨基酸的转运蛋白，我们已经确定了其他几个类似的转运蛋白作为疫苗的候选者。我们已经证明，当弯曲杆菌在鸡的肠道中时，这些表达。我们将创造疫苗，沙门氏菌表达这些其他运输蛋白，并测试它们的保护作用。CjaA疫苗有效的原因尚不清楚。用于将CjaA呈递给鸡疫苗的沙门氏菌菌株仍然具有一些可能驱动免疫发展的毒力特性。我们将研究沙门氏菌疫苗株的作用，不同的毒力，在针对CjaA的免疫反应的发展。了解沙门氏菌毒力特性在疫苗功能中的作用将有助于开发控制弯曲杆菌的改进策略。与BB/D 000866/1接合。

项目成果

Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome.

• DOI: 10.1099/mic.0.050278-0
• 发表时间: 2011-10
• 期刊: Microbiology (Reading, England)
• 作者: Chaudhuri RR;Yu L;Kanji A;Perkins TT;Gardner PP;Choudhary J;Maskell DJ;Grant AJ
• 通讯作者: Grant AJ