Post-translational regulation of CYP17 activity

描述（由申请人提供）：多囊卵巢综合征（PCOS）是育龄妇女最常见的生殖内分泌疾病。大约四分之三的无排卵性不孕症妇女患有PCOS，因此约占继发性闭经妇女的三分之一和约90%的月经过少妇女。多囊卵巢综合征的其他后果是多毛症，复发性早期妊娠丢失的发生率显着增加，估计50-61岁之间心肌梗死的风险增加11倍，年轻时子宫内膜癌的风险增加。在患有PCOS的女性中一致的发现是卵巢产生异常高的雄激素。有很好的证据表明，雄激素升高干扰了优势卵泡的选择，导致PCOS。雄激素生物合成所需的关键酶被称为CYP 17。该酶具有两种活性，其中之一，C17-20裂解酶活性，通过磷酸化增加。磷酸化位点尚未确定，并且关于磷酸化如何增加C17-20裂解酶活性知之甚少。在初步研究中，我们已经证实磷酸化增加C17-20裂解酶活性，并且有证据支持多囊卵巢中CYP 17磷酸化增加的假设。本项目的目的是鉴定CYP 17上的磷酸化位点，鉴定调节CYP 17磷酸化的激酶和磷酸酶，开始鉴定调节CYP 17磷酸化的细胞内信号传导机制和刺激它们的激素，并确定这些机制在卵巢高雄激素血症中的作用。为了实现这些目标，我们将使用分子建模技术来预测和优先考虑磷酸化位点和激酶识别位点。然后，我们将使用质谱、定点诱变和细胞表达技术来确认这些位点的身份。从这些实验的结果中，我们将通过确定磷酸化对底物和活性位点中产物对接的影响来完善分子模型。这些实验的结果将使我们能够确定在活性位点和其他位置的CYP 17和细胞内信号系统调节CYP 17磷酸化的治疗干预的目标。我们期望利用该分子模型设计新的药物，不仅治疗卵巢高雄激素血症，而且治疗其他雄激素过多的疾病，如前列腺癌和心血管疾病。

DESCRIPTION (provided by applicant): Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disease in women of reproductive age. Approximately three-quarters of women with anovulatory infertility have PCOS, thus accounting for approximately one-third of women with secondary amenorrhea and approximately 90% of women with oligomenorrhea. Other consequences of PCOS are hirsutism, markedly increased incidence of recurrent early pregnancy loss, an estimated 11-fold increased risk of myocardial infarction between the ages of 50-61 years, and an increased risk of endometrial cancer at a young age. A consistent finding in women with PCOS is that the ovaries produce abnormally high amounts of androgens. There is good evidence to conclude that elevated androgens interfere with selection of dominant follicles and cause PCOS. The key enzyme required for androgen biosynthesis is known as CYP17. The enzyme has two activities, one of which, the C17-20 lyase activity, is increased by phosphorylation. The phosphorylation sites have not been identified and there is little known about how phosphorylation increases C17-20 lyase activity. In preliminary studies, we have confirmed that phosphorylation increases C17-20 lyase activity and that there is evidence to support the hypothesis that CYP17 phosphorylation is increased in polycystic ovaries. The purpose of this project is to identify the phosphorylation sites on CYP17, to identify kinases and phosphatases that regulate CYP17 phosphorylation, to begin to identify intracellular signaling mechanisms that regulate CYP17 phosphorylation and the hormones that stimulate them, and to determine the role of these mechanisms in ovarian hyperandrogenism. To accomplish these goals we will use molecular modeling techniques to predict and prioritize phosphorylation sites and kinase recognition sites. We will then confirm the identity of these sites using mass spectrometry, site-directed mutagenesis and cellular expression techniques. From the results of these experiments we will refine the molecular model by determining the effects of phosphorylation on substrate and product docking in the active site. The results of these experiments will enable us to identify targets at the active site and other locations on CYP17 and in the intracellular signaling systems regulating CYP17 phosphorylation for therapeutic intervention. We expect to utilize the molecular model to design new Pharmaceuticals to treat not only ovarian hyperandrogenism, but also other diseases of androgen excess such as prostate cancer and cardiovascular diseases.