Analysis of proteins regulating polar flagellum localisation and number in Vibrio cholerae

霍乱弧菌极鞭毛定位和数量调节蛋白分析

• 批准号: BB/D003733/1
• 负责人: Gillian Fraser
• 金额: $ 26.56万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD003733%2F1
• 关键词: Analysis; proteins; regulating; polar; flagellum

The bacterium Vibrio cholerae causes worldwide epidemics and pandemics of the severe diarrhoeal disease cholera. V. cholerae swims through liquids by rotating a long corkscrew-like propeller, called a flagellum, which is an important factor in the ability of the bacterium to colonise the human host and cause disease. The flagellum is positioned at one end, or pole, of the cell, as is the machinery that exports the major virulence factor cholera toxin. To ensure that each V. cholerae cell has a single flagellum at one pole, the cell must carefully regulate when and where this structure is built. I aim to establish a project that will address two questions: How is the flagellum specifically targeted to one cell pole? How is the number of flagella per cell regulated? I will build on preliminary work to determine the functions of two proteins, FlhF and FlhG, that target the flagellum to the right place in the cell and regulate flagellar number, respectively. Through our studies, we will build up a picture of the molecular networks that link flagellum biogenesis, cell asymmetry (or polarity), and cell division.

细菌霍乱弧菌引起严重的肠道疾病霍乱的世界范围的流行和大流行。霍乱弧菌通过旋转一个长的螺旋桨在液体中游动，螺旋桨被称为鞭毛，鞭毛是细菌在人类宿主中定居并引起疾病的重要因素。鞭毛位于细胞的一端或极点，这是输出主要毒力因子霍乱毒素的机器。为了确保每个霍乱弧菌细胞在一个极点有一个鞭毛，细胞必须仔细调节这种结构的建立时间和地点。我的目标是建立一个项目，将解决两个问题：鞭毛是如何专门针对一个细胞极？每个细胞的鞭毛数量是如何调节的？我将建立在初步的工作，以确定两个蛋白质，FlhF和FlhG，目标鞭毛在细胞中的正确位置和调节鞭毛的数量，分别的功能。通过我们的研究，我们将建立一个分子网络的图片，链接鞭毛生物发生，细胞不对称性（或极性），和细胞分裂。