Analysis of co-translationally acting factors regulating the biogenesis of chloroplast encoded proteins (A05)

调节叶绿体编码蛋白生物发生的共翻译作用因子分析（A05）

• 批准号: 317545338
• 金额: --
• 依托单位: Zentrum für Synthetische Mikrobiologie (SYNMIKRO)
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317545338?language=en
• 关键词: Analysis; co; translationally; acting; factors

We found that heat acclimation leads to remodelling of the synthesis of specific chloroplast-encoded photosystem II core proteins. Furthermore, we revealed a broad network of ribosome-associated factors in chloroplasts, containing many putative modulators and integrators with altered ribosome-binding behaviour during heat acclimation. Based on these findings, the proposed project aims to deepen the understanding how protein biogenesis is regulated during temperature acclimation. We plan to investigate selected putative modulators for their contribution for tuning translation itself and to examine central steps of co-translational nascent polypeptide processing and membrane integration.

我们发现热驯化会导致特定叶绿体编码的光系统 II 核心蛋白的合成重塑。此外，我们揭示了叶绿体中核糖体相关因子的广泛网络，其中包含许多假定的调节剂和整合剂，在热适应过程中核糖体结合行为发生改变。基于这些发现，拟议的项目旨在加深对温度适应过程中蛋白质生物合成如何调节的理解。我们计划研究选定的假定调节剂对调节翻译本身的贡献，并检查共翻译新生多肽加工和膜整合的核心步骤。