GLUCOREGULATORY PEPTIDE HORMONES

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这些研究（R 01 DK 002700）的总体目标旨在确定脂肪酸（FFA）如何在肥胖早期刺激β细胞代偿，但在肥胖晚期导致代偿β细胞功能障碍和死亡以及NIDDM。我们还对FFA对远端组织的直接影响感兴趣;特别是储存的甘油三酯如何影响心脏功能。这项研究的目的是开发和验证质子磁共振波谱（1H MRS）作为体内工具来测量心肌脂质含量。在大鼠组织离体和在15个健康的人在体内的补充研究提供的证据表明，1H MRS构成了一个可重复的技术测量心肌甘油三酯。在Zucker大鼠心肌组织中，甘油三酯的1H MRS测量结果与生化测量结果相匹配（P 0.001）。在人类受试者中，甘油三酯在非常瘦的个体的心脏中是明显的，并且在超重和肥胖受试者中升高。通过心脏成像测量，心肌甘油三酯含量增加伴有LV质量升高和间隔壁增厚抑制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The general goal of these studies (R01DK002700) are designed to determine how fatty acids (FFA) stimulate beta-cell compensation early in obesity and yet cause dysfunction and death of compensated beta-cells and NIDDM late in obesity. We are also interested in the direct effects of FFA on remote tissues; particularly how stored triglycerides impact cardiac function. The goal of this recent study was to develop and validate proton magnetic resonance spectroscopy (1H MRS) as an in vivo tool to measure myocardial lipid content. Complementary studies in rat tissue ex vivo and in 15 healthy humans in vivo provided evidence that 1H MRS constitutes a reproducible technique for the measurement of myocardial triglyceride. In myocardial tissue from Zucker rats, the 1H MRS measurement of triglyceride matched that obtained by biochemical measurement (P 0.001). In human subjects triglyceride was evident in the hearts of even the very lean individuals and was elevated in overweight and obese subjects. Increased myocardial triglyceride content was accompanied by elevated LV mass and suppressed septal wall thickening as measured by cardiac imaging.