STUDY OF C ELEGANS BY FRET AND FCS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。使用FRET显微镜进行了秀丽隐杆线虫中整合素连接激酶（ILK）蛋白相互作用的体内研究（Breusegem等，2002），结果支持使用FRET显微镜更详细地研究相互作用的可能性。在这项工作中，在体内研究ILK相互作用在成年C Elegans使用FRET显微镜继续和ILK蛋白在胚胎状态下的肌肉结构的形成过程中的相互作用将使用FRET显微镜和荧光相关光谱检查。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In vivo study of Integrin-Linked Kinase (ILK) proteins interactions in C Elegans using FRET microscopy was performed (Breusegem, et al 2002) and the results support the possibility of using FRET microscopy to study the interactions more in detail. In this work, the in vivo study of ILK interactions in adult C Elegans using FRET microscopy is continued and the interactions of the ILK proteins during the formation of muscle structure in the embryonic state will be examined using FRET microscopy and fluorescence correlation spectroscopy.