ROLE OF CYPA AS A MOLECULAR IMAGING OR THERAPEUTIC TARGET

CYPA 作为分子成像或治疗靶点的作用

• 批准号: 7358266
• 负责人: EDWARD F PATZ
• 金额: $ 0.51万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358266
• 关键词: ROLE; CYPA; MOLECULAR; IMAGING; THERAPEUTIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The role of Cyclophilin A (CypA) is unknown in cancer. Using lung cancer cells which have CypA expression knocked down and control cells with endogenous CypA levels, we have performed in vitro assays and are completing in vivo assays of tumorigenesis with subcutaneous xenografts in SCID mice. This protein may be a useful target for molecular imaging and therapeutics. However, given the unknown role of CypA, it is crucial that we use available resources to get as much data as possible beyond these traditional methods. The high quality PET imaging equipment in the Duke Center for In Vivo Microscopy will allow us to examine the in vivo role of CypA in ways complementary to traditional pathology. Using FDG-PET, we may compare tumor size, function, and metabolic activity between knockdown and control animals. We may also compare the presence and degree of metastases from the primary subcutaneous xenograft to distant sites between knockdown and control animals, an experiment uniquely suited to FDG-PET imaging. The procedure entails a tail vein injection of FDG, and a PET scan, is relatively non-invasive, and should not cause undue harm to the animal

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。亲环素A（CypA）在癌症中的作用尚不清楚。使用CypA表达被敲低的肺癌细胞和具有内源性CypA水平的对照细胞，我们已经进行了体外测定，并且正在完成SCID小鼠皮下异种移植物的肿瘤发生的体内测定。该蛋白可能是分子成像和治疗的有用靶点。然而，考虑到CypA的未知作用，我们必须利用现有资源，在这些传统方法之外获得尽可能多的数据。杜克体内显微镜中心的高质量PET成像设备将使我们能够以与传统病理学互补的方式检查CypA的体内作用。 使用FDG-PET，我们可以比较敲除和对照动物之间的肿瘤大小，功能和代谢活性。我们还可以比较敲除动物和对照动物之间从原发性皮下异种移植物到远处部位的转移的存在和程度，这是一种独特地适合于FDG-PET成像的实验。该过程需要尾静脉注射FDG和PET扫描，相对无创，不会对动物造成不必要的伤害