ACETYLCHOLINE BINDING PROTEIN & ACETYLCHOLINE ESTERASE

乙酰胆碱结合蛋白

• 批准号: 7358062
• 负责人: PALMER William TAYLOR
• 金额: $ 1.02万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358062
• 关键词: ACETYLCHOLINE; BINDING; PROTEIN; ESTERASE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have have prepared the extracellular domain of the nicotinic acetylcholine receptor by synthesizing a gene homologous with the snail binding protein. It shows the appropriate ligand specificity and hydrodynamic properties, but we would like to check to see whether it assembles as a circular pentamer rather than a linear tetramer through hexamer. Negative staining of the protein on a grid may resolve this issue.Part BSome years ago Dr Ellisman's and my laboratory employed selective immunogold labeling to localize acetylcholinesterase in the synapse. The tools for localization have greatly improved aver the years, and we now have a small peptide, fasciculin, that interacts with acetylcholinesterase at low pM concentrations. The Ellisman laboratory has developed a novel eosin labeling technique that greatly improves the resolution of deposition of electron dense materal. Hence, we hope to employ eosin-labeled fasciculin to localize synaptic acetylcholinesterase with higher resolution chemistry. Our laboratory has the materials and skills to make labeled eosi

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。我们已经通过与蜗牛结合蛋白合成基因同源物来制备烟碱乙酰胆碱受体的细胞外结构域。它显示了适当的配体特异性和流体动力特性，但我们想检查一下它是否作为圆形的五聚体组装而不是线性四聚体，而不是通过己酰胺剂组装。蛋白质在网格上的负染色可能可以解决此问题。几年前，Ellisman博士和我的实验室采用了选择性的免疫金标记，将乙酰胆碱酯酶定位在突触中。多年来的定位工具已经大大改善了平均水平，现在我们有一个较小的肽筋膜，该肽与乙酰胆碱酯酶在低PM浓度下与乙酰胆碱酯酶相互作用。 Ellisman实验室开发了一种新型的曙红标记技术，可大大改善电子致密材料沉积的分辨率。因此，我们希望采用烯烃标记的筋膜来将突触乙酰胆碱酯酶与较高的分辨率化学定位。我们的实验室具有制作标签EOSI的材料和技能