CRYSTAL COLLECTION OF BAK AND CCBL UBA DOMAIN

BAK 和 CCBL UBA 领域的水晶收藏

• 批准号: 7357734
• 负责人: KALLE B GEHRING
• 金额: $ 1.33万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357734
• 关键词: CRYSTAL; COLLECTION; BAK; CCBL; UBA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bcl-2 homologous antagonist killer (Bak) is a pro-apoptotic member of the B cell lymphoma (Bcl) family of proteins which is directly implicated in many pathologies related to aberant apoptosis (e.g. cancer and neurodegeneration). Any structural information related to Bak is crucial for the understanding of its functional role and we have obtained a previous structure and would like to follow up with a derivatized Bak crystal that diffracts on our home source at best at 3A. We hope to bring the resolution `down` to improve the refinement statistics. cCBL and CBL-b are adaptors for the tyrosine kinase receptors (Met receptors - cancer related), which contain a Ubiquitin E3 ligase domain and a C-terminal UBA domain. Interestingly, c-CBL UBA does not bind ubiquitin whereas the CBL-b UBA does.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。Bcl-2同源拮抗剂杀伤（巴克）是蛋白质的B细胞淋巴瘤（Bcl）家族的促凋亡成员，其直接涉及与异常凋亡相关的许多病理（例如癌症和神经变性）。与巴克相关的任何结构信息对于理解其功能作用至关重要，我们已经获得了先前的结构，并希望继续使用衍生的巴克晶体，该晶体在我们的原始来源上衍射最多为3 A。我们希望降低分辨率以改善细化统计数据。 cCBL和CBL-b是酪氨酸激酶受体（Met受体-癌症相关）的衔接子，含有泛素E3连接酶结构域和C-末端乌巴结构域。有趣的是，c-CBL乌巴不结合泛素，而CBL-b乌巴结合。