CRYSTAL COLLECTION OF BAK AND CCBL UBA DOMAIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 BCL-2同源拮抗剂杀手(Bak)是B细胞淋巴瘤(Bcl1)家族中促凋亡蛋白家族的一员，直接参与许多与Aberant细胞凋亡相关的病理过程(如癌症和神经变性)。任何与Bak相关的结构信息对了解其功能作用都是至关重要的，我们已经获得了以前的结构，并希望后续推出衍生化Bak晶体，该晶体最多在3A的情况下对我们的原生源进行衍射。我们希望降低决议，以改进精细化统计数据。CCBL和CBL-b是酪氨酸激酶受体(Met受体与癌症相关)的适配子，它含有一个泛素E3连接酶结构域和一个C端UBA结构域。有趣的是，c-CBL UBA不结合泛素，而CBL-b UBA则结合泛素。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bcl-2 homologous antagonist killer (Bak) is a pro-apoptotic member of the B cell lymphoma (Bcl) family of proteins which is directly implicated in many pathologies related to aberant apoptosis (e.g. cancer and neurodegeneration). Any structural information related to Bak is crucial for the understanding of its functional role and we have obtained a previous structure and would like to follow up with a derivatized Bak crystal that diffracts on our home source at best at 3A. We hope to bring the resolution `down` to improve the refinement statistics. cCBL and CBL-b are adaptors for the tyrosine kinase receptors (Met receptors - cancer related), which contain a Ubiquitin E3 ligase domain and a C-terminal UBA domain. Interestingly, c-CBL UBA does not bind ubiquitin whereas the CBL-b UBA does.