NMR STUDIES ON BRAIN METABOLISM

脑代谢的核磁共振研究

• 批准号: 7420598
• 负责人: ANITA SIDHU
• 金额: $ 0.92万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-20 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420598
• 关键词: Krebs' cycle; alanine; arginine; aspartate; brain metabolism; citrulline; dopamine; glutamates; glutamine; lactates; measurement; metabolism; nitric oxide; nucleotides; phenylalanine; succinates; tyrosine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brain metabolism is being studied using double labelled 13C-14N-glutamine to label TCA cycle metabolites including citrate, fumarate and succinate, in addition to TCA by-products including aspartate, glutamate, phenylalanine and tyrosine as well as alanine and lactate. Cells were treated with dopamine and dopamine agonits to study the effects of dopamine on citotoxic events in the cells. Additional measurements of citrulline and arginine will be performed as a indication of Nitric oxide metabolism and labeling of purine nucleotides with 14N (as glutamine is an immediated precursor of these nucleotides) will make possible the measurements of nucleotides in extracted cells.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。除了在包括天冬氨酸，谷氨酸，谷氨酸，苯丙氨酸和酪氨酸以及丙氨酸和丙氨酸和丙氨酸和丙氨酸的TCA外，还研究了使用双重标记的13C-14N-谷氨酰胺研究脑代谢，以标记包括柠檬酸盐，富马酸盐和琥珀酸酯在内的TCA循环代谢产物。用多巴胺和多巴胺激动剂处理细胞，以研究多巴胺对细胞中的石质毒性事件的影响。瓜氨酸和精氨酸的其他测量将作为一氧化氮代谢的指示以及用14N的嘌呤核苷酸标记（因为谷氨酰胺是这些核苷酸的直接前体）将使提取细胞中核苷酸的测量值。