Analysis of the role of the TAT protein translocation system in Campylobacter jejuni

空肠弯曲菌TAT蛋白易位系统的作用分析

• 批准号: BB/D008395/1
• 负责人: Dave Kelly
• 金额: $ 31.46万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD008395%2F1
• 关键词: Analysis; role; TAT; protein; translocation

Some bacteria get into the food that we eat and cause food-poisoning. Some of these bacteria are quite common but are usually killed during cooking. When chicken meat is not cooked properly, one of these types of bacteria, called Campylobacter jejuni, is a particular problem. If we can understand what allows C.jejuni to grow in chickens and reduce the level of contamination of chicken carcasses, this will help reduce the scale of the risk to human health. In the chicken gut, the oxygen concentrations are very low and C. jejuni has to adapt it's metabolism to this environment. This project is aimed at understanding how certain proteins which are needed in this adaptation and which often function outside the cell, get to their final location using a special type of export system. We will try to find out what happens when the cell is prevented from exporting these proteins, and what the functions of some of them are. The results should help us to understand better the role that these types of exported proteins play in the overall biology of campylobacter and may even identify specific targets for intervention that might in the future allow the control of the growth of the bacteria in the avian gut.

有些细菌进入我们吃的食物，引起食物中毒。其中一些细菌很常见，但通常在烹饪过程中被杀死。当鸡肉没有正确烹饪时，其中一种称为空肠弯曲杆菌的细菌是一个特别的问题。如果我们能够了解是什么让空肠弯曲菌在鸡体内生长，并降低鸡尸体的污染水平，这将有助于降低对人类健康的风险。在鸡肠道中，氧浓度很低，C。空肠的新陈代谢必须适应这种环境。该项目旨在了解这种适应所需的某些蛋白质以及通常在细胞外发挥作用的蛋白质如何使用特殊类型的输出系统到达其最终位置。我们将试图找出当细胞被阻止输出这些蛋白质时会发生什么，以及其中一些蛋白质的功能是什么。这些结果应该有助于我们更好地了解这些类型的输出蛋白在弯曲杆菌的整体生物学中所起的作用，甚至可以确定特定的干预目标，这些目标可能在未来允许控制细菌在禽类肠道中的生长。

项目成果

It takes two to tango: two TatA paralogues and two redox enzyme-specific chaperones are involved in the localization of twin-arginine translocase substrates in Campylobacter jejuni.

• DOI: 10.1099/mic.0.080713-0
• 发表时间: 2014-09
• 期刊: Microbiology (Reading, England)
• 作者: Liu YW;Hitchcock A;Salmon RC;Kelly DJ
• 通讯作者: Kelly DJ