The enigma of oxygen intolerance in Campylobacter jejuni: an integrative transcriptomic proteomic and physiological approach

空肠弯曲杆菌氧不耐受之谜：一种整合的转录组、蛋白质组和生理学方法

• 批准号: BB/E014240/1
• 负责人: Dave Kelly
• 金额: $ 43.78万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE014240%2F1
• 关键词: enigma; oxygen; intolerance; Campylobacter; jejuni

Some bacteria get into the food that we eat and cause food-poisoning. Some of these bacteria are quite common but are usually killed during cooking. When chicken meat is not cooked properly, one of these types of bacteria, called Campylobacter jejuni, is a particular problem. If we can understand what allows C.jejuni to grow in chickens and reduce the level of contamination of chicken carcasses, this will help reduce the scale of the risk to human health. In the chicken gut, the oxygen concentrations are very low and C. jejuni has to adapt it's metabolism to this environment. This project is aimed at understanding how this happens and what genes and proteins are important in regulating this process. We will be looking at how the bacterium copes with low oxygen concentrations on the one hand, but also how it resists the potentially damaging effects of oxidative stress when it comes into contact with high oxygen levels. We will do this by using special continuous growth techniques in an apparatus called a fermenter. The results should help us to identify specific genes or proteins that are important in growth at high or low oxygen levels and these might be targets for intervention that might in the future allow the control of the growth of the bacteria in the chicken gut.

一些细菌进入我们吃的食物中，导致食物中毒。其中一些细菌很常见，但通常会在烹饪过程中被杀死。当鸡肉没有被正确煮熟时，其中一种被称为空肠弯曲杆菌的细菌就会成为一个特别的问题。如果我们能够了解是什么允许空肠弯曲菌在鸡体内生长，并降低鸡尸的污染水平，这将有助于降低对人类健康的风险规模。在鸡的肠道中，氧气浓度非常低，空肠弯曲菌必须适应这种环境的代谢。该项目旨在了解这一过程是如何发生的，以及哪些基因和蛋白质在调节这一过程中起着重要作用。我们将研究细菌如何应对低氧浓度，但当它与高氧水平接触时，也将如何抵抗氧化应激的潜在破坏性影响。我们将通过在一种称为发酵罐的设备中使用特殊的连续生长技术来实现这一点。这些结果应该有助于我们识别在高或低氧水平下对生长至关重要的特定基因或蛋白质，这些基因或蛋白质可能是未来可能控制鸡肠道细菌生长的干预目标。

项目成果

Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability.

• DOI: 10.1111/1462-2920.13930
• 发表时间: 2017-10
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Guccione EJ;Kendall JJ;Hitchcock A;Garg N;White MA;Mulholland F;Poole RK;Kelly DJ
• 通讯作者: Kelly DJ

Hemerythrins in the microaerophilic bacterium Campylobacter jejuni help protect key iron-sulphur cluster enzymes from oxidative damage.

• DOI: 10.1111/1462-2920.12341
• 发表时间: 2014-04
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Kendall JJ;Barrero-Tobon AM;Hendrixson DR;Kelly DJ
• 通讯作者: Kelly DJ

Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch.

• DOI: 10.1099/mic.0.021790-0
• 发表时间: 2009
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: John A. Wright;A. Grant;D. Hurd;M. Harrison;Edward Guccione;D. Kelly;D. Maskell