MICROBIAL DIVERSITY IN SEDIMENT

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。我正在研究来自不同水体（目前是Mount Hope Bay）的沉积物样本中的微生物多样性。我使用BRC纳米滴来测定PCR反应后针对16s RNA的V6区域的DNA量。我们在大厅对面有一台光谱仪，但我往往得到不同的结果，并且从其他人那里听说纳米滴是多么可靠。我的资金来源是美国国家航空和宇宙航行局给私家侦探Mitch Sogin的天体生物学资助

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I am studying microbial diversity in sediment samples from various bodies of water (currently Mount Hope Bay). I used the BRC nanodrop to determine the amount of DNA after a PCR reaction targeting the V6 region of 16s RNA. We have a spectrometer across the hall but I tend to get variable results and had heard from others how reliable the nanodrop was. My source of funding is an Astrobiology grant from NAI/NASA to P.I. Mitch Sogin