MICROBIAL DIVERSITY IN SEDIMENT

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。我正在研究不同水体(目前的希望山湾)沉积物样本中的微生物多样性。在针对16S RNA的V6区域进行PCR反应后，我使用了BRC纳米滴来确定DNA的数量。我们在大厅对面有一台光谱仪，但我得到的结果往往是不同的，我从其他人那里听说纳米液滴是多么可靠。我的资金来源是NAI/NASA向P.I.米奇·索金提供的天体生物学赠款

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I am studying microbial diversity in sediment samples from various bodies of water (currently Mount Hope Bay). I used the BRC nanodrop to determine the amount of DNA after a PCR reaction targeting the V6 region of 16s RNA. We have a spectrometer across the hall but I tend to get variable results and had heard from others how reliable the nanodrop was. My source of funding is an Astrobiology grant from NAI/NASA to P.I. Mitch Sogin