GLIAL MEMBRANES AT THE NODE OF RANVIER PREVENT NEURITE OUTGROWTH

RANVIER结处的胶质膜防止神经突生长

• 批准号: 7420807
• 负责人: DAVID R COLMAN
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420807
• 关键词: axon; central nervous system; culture; dendrites; myelin; myelin glycoprotein; neuronal guidance; neurons; node of Ranvier; oligodendroglia; proteomics; spinal cord; thinking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp-null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。朗飞结是沿着有髓神经规则排列的无髓轴突节段。在这里，我们发现从哺乳动物中枢神经系统（CNS）中分离出的结膜限制了培养神经元的神经突生长。这些膜的蛋白质组学分析揭示了神经突生长的几种抑制剂，包括少突胶质细胞髓磷脂糖蛋白（OMgp）。在大鼠脊髓中，OMgp 并不像以前认为的那样定位于致密髓磷脂，而是定位于少突胶质细胞，其过程会聚形成完全包围节点的环。在 OMgp 缺失小鼠中，中枢神经系统节点异常宽，并且观察到侧枝发芽。中枢神经系统中的节点鞘可以稳定节点并防止轴突发芽。