ACTIN-BINDING PROTEINS IN A POSTSYNAPTIC PREPARATION: LASP-1 IS A COMPONENT OF

突触后制剂中的肌动蛋白结合蛋白：LASP-1 是

• 批准号: 7420656
• 负责人: DAVID R COLMAN
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420656
• 关键词: actins; polymerization; proteins; synapses

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. CNS synapses are complex sites of cell-cell communication. Identification and characterization of the protein components of synapses will lead to a better understanding of the mechanisms of neurotransmission and plasticity. We applied multidimensional protein identification technology (MudPIT) to purified, guanidine-solubilized postsynaptic fractions to identify novel synaptically localized molecules. We identified several actin-associated proteins known to regulate actin polymerization and control cell motility in nonneural cells that have not previously been associated with CNS synaptic function. One of these is lasp-1, an actin-associated LIM and SH3 domain-containing protein. We show that lasp-1 is strongly expressed by CNS neurons and is concentrated at synaptic sites. Overall, the preponderance of actin-associated proteins in postsynaptic density fractions, and specifically those involved in actin reorganization, suggests that there are many modes by which the state of synaptic F-actin polymerization and, hence, synaptic physiology are affected.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。中枢神经系统突触是细胞间通讯的复杂场所。识别和表征突触的蛋白质组分将导致更好地理解神经传递和可塑性的机制。我们应用多维蛋白质鉴定技术（MudPIT）纯化，胍溶解突触后馏分，以确定新的突触定位分子。我们确定了几个肌动蛋白相关的蛋白质，已知调节肌动蛋白聚合和控制细胞运动的非神经细胞，以前没有与中枢神经系统突触功能。其中之一是lasp-1，一种与肌动蛋白相关的LIM和含有SH 3结构域的蛋白质。我们发现，lasp-1是强烈表达的中枢神经系统神经元，并集中在突触部位。总的来说，肌动蛋白相关蛋白在突触后密度分数的优势，特别是那些参与肌动蛋白重组，表明有许多模式，突触F-肌动蛋白聚合的状态，因此，突触生理受到影响。