PROTEOMIC ANALYSIS OF MATURE MELANOSOMES FROM THE RETINAL PIGMENTED EPITHELIUM

视网膜色素上皮成熟黑素体的蛋白质组学分析

• 批准号: 7420738
• 负责人: David Collin Williams
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420738
• 关键词: enzymes; epithelium; immunoelectron microscopy; mass spectrometry; melanosomes; organelles; proteins; proteomics; retinal pigment epithelium

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The protein content of melanosomes in the retinal pigment epithelium (RPE) was analyzed by mass spectrometry. More than 100 proteins were found to be common to two out of three variations of sample preparation. Some proteins normally associated with other organelles were detected. Several lysosomal enzymes were detected, with the presence of cathepsin D confirmed by immunoelectron microscopy, thus supporting the previously suggested notion that melanosomes may contribute to the degradation of ingested photoreceptor outer segment disks.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。通过质谱分析了视网膜色素上皮（RPE）中黑素体的蛋白质含量。在样品制备的三种变体中，发现了100多种蛋白质是共同的。一些通常与其他细胞器相关的蛋白质。检测到几种溶酶体酶，通过免疫电子显微镜证实了组织蛋白酶D，因此支持先前建议的观念，即黑素体可能有助于降解摄入的光感受器外部段盘的降解。