IDENTIFICATION OF DMC1 INTERACTING PROTEINS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。以往对减数分裂重组复合体蛋白质组分的生化表征是有限的。在有丝分裂的酵母或大肠杆菌中表达后，只有少数重组复合体的成分被鉴定出来，这些成分仅在减数分裂背景之外进行了研究。我们正试图通过从正常细胞环境中纯化减数分裂重组复合体来鉴定和表征减数分裂重组复合体的成分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous biochemical characterization of the protein components of meiotic recombination complexes has been limited. Only a few components of the recombination complex have been identified and these have been studied only outside of a meiotic context following expression in mitotic yeast or E. coli. We are attempting to identify and characterize components of the meiotic recombination complex by purifying them from the normal cellular context in which they function.