BIOCHEMICAL ANALYSIS OF CENTROSOMAL PROTEIN COMPLEXES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。中心体是动物细胞中主要的微管组织中心。我们的实验室对确定中心体如何组装和功能感兴趣，使用秀丽隐杆线虫作为模型。许多实验室先前的工作已经确定了这种生物体中心体功能所需的约15种蛋白质。我们目前正在利用生物化学进一步表征这些蛋白质，并通过它们与已知中心体蛋白的相互作用来鉴定其他蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Centrosomes are the primary microtubule organizing centers in animal cells. Our laboratory is interested in determining how centrosomes assemble and function, using C. elegans as a model. Previous work in a number of laboratories has identified ~15 proteins required for centrosome function in this organism. We are currently using biochemistry to further characterize these proteins and identify additional proteins by virtue of their interaction with known centrosomal proteins.