BIOCHEMICAL ANALYSIS OF CENTROSOMAL PROTEIN COMPLEXES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 中心体是动物细胞中主要的微管组织中心。我们的实验室有兴趣确定中心体如何组装和功能，使用C。优雅的模特许多实验室的先前工作已经确定了该生物体中中心体功能所需的约15种蛋白质。我们目前正在使用生物化学来进一步表征这些蛋白质，并通过它们与已知中心体蛋白质的相互作用来鉴定其他蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Centrosomes are the primary microtubule organizing centers in animal cells. Our laboratory is interested in determining how centrosomes assemble and function, using C. elegans as a model. Previous work in a number of laboratories has identified ~15 proteins required for centrosome function in this organism. We are currently using biochemistry to further characterize these proteins and identify additional proteins by virtue of their interaction with known centrosomal proteins.