DIFFERENTIAL EFFECTS OF OSMOTIC COMPRESSION ON MYOFLIAMENT LATTICE STRUCTURES

渗透压对肌丝晶格结构的不同影响

• 批准号: 7369173
• 负责人: DAVID Wayne MAUGHAN
• 金额: $ 0.75万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369173
• 关键词: DIFFERENTIAL; EFFECTS; OSMOTIC; COMPRESSION; MYOFLIAMENT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Skinned muscle fibers have been used extensively for structure-function of muscle contraction. Methods of osmotic compression have been used to return the otherwise swollen skinned fibers to in vivo size (Kawai et al., 1993; Chinn et al., 2000). Complicating the issue, osmotic agents can affect internal structures differentially, thereby confounding the interpretation of structural and kinetic data. Dextran T500 (the agent used in most skinned fiber studies), for example, does not permeate the myofilament lattice, while Dextran T10 or PEG 4000 does. The former compresses the lattice and, presumably, the filaments within, whereas the latter do permeate the lattice and therefore compress the filaments but not the filament lattice. The objectives of the proposed experiments are to understand the range of structural effects at the lattice and filament levels resulting from osmotic compression of muscle fibers. We will also determine the differential changes in spacing, diameter, and mass ratio between thick and thin filaments as a function of compressive pressure. The results of the experiments will provide a structural basis for interpreting past and future functional studies investigating effects of osmotic compression on kinetic properties of muscle. (Experiments will be done March 2006)

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。皮肤肌纤维在肌肉收缩的结构和功能方面有着广泛的应用。渗透压的方法被用来将原本肿胀的皮肤纤维恢复到体内大小（Kawai等人，1993；Chinn等人，2000）。使问题复杂化的是，渗透剂会对内部结构产生不同的影响，从而混淆了结构和动力学数据的解释。例如，右旋糖酐T500（在大多数剥皮纤维研究中使用的药物）不能渗透肌丝晶格，而右旋糖酐T10或PEG 4000可以。前者压缩了晶格，可能也压缩了其中的细丝，而后者确实渗透到晶格中，因此压缩了细丝而不是细丝晶格。所提出的实验的目的是了解在晶格和细丝水平上的结构效应的范围，这是由肌肉纤维的渗透压缩引起的。我们还将确定粗细丝和细细丝之间的间距、直径和质量比的差异变化作为压缩压力的函数。实验结果将为解释过去和未来关于渗透压缩对肌肉动力学特性影响的功能研究提供结构基础。（实验将于2006年3月进行）