STRUCTURE & FUNCTION RELATIONSHIP: PROSTANOID RECEPTORS
结构
基本信息
- 批准号:7325742
- 负责人:
- 金额:$ 35.32万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-12-01 至 2009-11-30
- 项目状态:已结题
- 来源:
- 关键词:Affinity LabelsAgonistBindingBiochemicalBiologicalBiological AssayBiological ProcessBiologyBlood VesselsCalciumCircular DichroismConstriction procedureCoupledCouplingCyclic AMPDinoprostDinoprostoneEndotheliumEpoprostenolEpoprostenol ReceptorsExtracellular DomainFamilyFluorescence SpectroscopyG-Protein-Coupled ReceptorsGTP-Binding ProteinsGoalsHemodynamic ProcessesHemostatic functionHomeostasisHumanInflammationInterventionLigand BindingLigandsMediatingMediationMediator of activation proteinMembraneModelingMolecularMolecular ConformationMutagenesisMyocardial InfarctionNMR SpectroscopyNuclear Magnetic ResonanceNumbersPGF receptorPathologic ProcessesPeptide SynthesisPhysiologicalPlatelet Aggregation InhibitorsPlatelet aggregationPlayProductionProstaglandin D2Prostaglandin E ReceptorProstaglandinsProstaglandins IReceptor SignalingRecombinant ProteinsRecombinantsRelaxationResearchResearch PersonnelResolutionRoleSignal TransductionSiteSite-Directed MutagenesisSmooth MuscleSolutionsSpecificityStrokeStructureStructure-Activity RelationshipTechniquesTestingThrombosisThromboxane A2Thromboxane A2 ReceptorTissuesTransmembrane DomainVascular DiseasesVascular Endothelial CellVascular Smooth MuscleVasoconstrictor AgentsVasodilator Agentsaffinity labelingbaseconceptdesigndisulfide bondextracellularhuman diseasehuman prostaglandin D2 receptorinterestmolecular modelingmutantpeptide structureprogramsprostaglandin D receptorprostaglandin EP3 receptorreceptorresearch studysynthetic peptidethree dimensional structure
项目摘要
DESCRIPTION (provided by applicant): The long-term goal of this project is to understand how prostanoid receptors mediate human physiopathological vascular and hemodynamic processes, including hemostasis, thrombosis, and inflammation. Prostanoids, including thromboxane A2 (TXA2), prostacyclin 12 (PGI2) and prostaglandin D2 (PGD2), E2 (PGE2) and F2 (PGF2) are synthesized by vascular smooth muscle, endothelium, and other tissues. TXA2 is a potent stimulator of platelet aggregation and a smooth muscle constrictor. PGI2 actions are essentially opposite to those of TXA2. PGE2 plays diverse/opposite functions as a vasodilator or vasoconstrictor based on the mediations of the subtype receptors. TXA2 and PGI2 are also mediated by their receptors. All the receptors belong to the G protein-coupled receptor family, with seven transmembrane domains, and are coupled to different signaling. The structure and function relationships of the TXA2 receptor (TP), the PGI2 receptor (IP) and four subtype PGE2 receptors (EP) are poorly defined, with little structural information on how the prostanoids recognize their receptors and signaling through G proteins specifically. The diverse receptor-mediated actions of TXA2, PGI2, and PGE2 have led us to hypothesize that the receptors have distinct 3D structures in their extracellular ligand recognition sites, and in their intracellular G protein-coupled sites. Recently, we have partially characterized the ligand-binding pockets in the extracellular domains, and parts of the intracellular G protein coupling domains of the TP and IP receptors. Further characterization of the specific ligand recognition and G protein coupling sites of the TP, IP, and a typical EP receptor should reveal their differences. The following specific aims will use recombinant receptor mutants, synthetic peptides, circular dichroism and 2D nuclear magnetic resonance spectroscopy to: 1. Identify the segments and key residues comprising the TP receptor agonist recognition pocket for comparison with the antagonist recognition pocket; 2. Determine the solution conformation of the extracellular domains of the IP receptor and define the segments and key residues making up the ligand recognition pocket; 3. Determine the solution conformation of the extracellular domains in the human EP3 receptor, and identify the residues making up its specific ligand recognition pocket for comparison with those of the TP and IP receptors; 4. Determine the solution conformation of segments comprising the intracellular domains.
描述(由申请人提供):本项目的长期目标是了解前列腺素受体如何介导人类生理病理学血管和血液动力学过程,包括止血、血栓形成和炎症。前列腺素,包括血栓素A2(TXA 2)、前列环素12(PGI 2)和前列腺素D2(PGD 2)、E2(PGE 2)和F2(PGF 2),由血管平滑肌、内皮和其它组织合成。TXA 2是血小板聚集的有效刺激剂和平滑肌收缩剂。PGI 2的作用与TXA 2相反。基于亚型受体的介导,PGE 2作为血管扩张剂或血管收缩剂发挥不同/相反的功能。TXA_2和PGI_2也是由它们的受体介导的。所有的受体都属于G蛋白偶联受体家族,具有七个跨膜结构域,并且与不同的信号传导偶联。TXA 2受体(TP)、PGI 2受体(IP)和四种亚型PGE 2受体(EP)的结构和功能关系尚不清楚,关于前列腺素类如何识别其受体和特异性通过G蛋白信号传导的结构信息很少。TXA 2、PGI 2和PGE 2的不同受体介导的作用使我们假设这些受体在其细胞外配体识别位点和细胞内G蛋白偶联位点具有不同的3D结构。最近,我们已经部分地表征了TP和IP受体的胞外结构域中的配体结合口袋和部分胞内G蛋白偶联结构域。TP、IP和典型EP受体的特异性配体识别和G蛋白偶联位点的进一步表征应揭示它们的差异。以下具体目的将利用重组受体突变体、合成肽、圆二色性和二维核磁共振谱来:1.鉴定组成TP受体激动剂识别口袋的片段和关键残基,用于与拮抗剂识别口袋进行比较; 2.确定IP受体胞外结构域的溶液构象,并确定组成配体识别口袋的片段和关键残基; 3.确定人EP 3受体胞外结构域的溶液构象,并鉴定组成其特异性配体识别口袋的残基,以与TP和IP受体的残基进行比较; 4.确定包含胞内结构域的片段的溶液构象。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Involvement of non-conserved residues important for PGE2 binding to the constrained EP3 eLP2 using NMR and site-directed mutagenesis.
- DOI:10.1016/j.febslet.2008.07.018
- 发表时间:2008-08-20
- 期刊:
- 影响因子:3.5
- 作者:Chillar, Annirudha;Wu, Jiaxin;So, Shui-Ping;Ruan, Ke-He
- 通讯作者:Ruan, Ke-He
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KE-HE RUAN其他文献
KE-HE RUAN的其他文献
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{{ truncateString('KE-HE RUAN', 18)}}的其他基金
Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
前列腺素 I 合酶、血栓素 A 合酶
- 批准号:
7820930 - 财政年份:2009
- 资助金额:
$ 35.32万 - 项目类别:
Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
前列腺素 I 合酶、血栓素 A 合酶
- 批准号:
7446366 - 财政年份:1996
- 资助金额:
$ 35.32万 - 项目类别:
PGI & TXA SYNTHASES--MEMBRANE ANCHOR STRUCTURE/FUNCTION
前列腺素I
- 批准号:
2460216 - 财政年份:1996
- 资助金额:
$ 35.32万 - 项目类别:
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