ULTRA-HIGH RESOLUTION STRUCTURES OF THE SRP GTPASE AND ITS HETERODIMERIC RECEPTO

SRP GTPase 及其异二聚体受体的超高分辨率结构

• 批准号: 7370355
• 负责人: Douglas M. Freymann
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370355
• 关键词: ULTRA; RESOLUTION; STRUCTURES; SRP; GTPASE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal has two aims: the first is completion of ultra-high resolution data collection from the GDP and GMPPNP complexes of the SRP GTPase, Ffh, from Thermus aquaticus. These structures, with data from crystals of the apo protein previously collected at SSRL, will form the basis for a comparitive analysis of the structural states of Ffh at ultra-high resolution. Although a member of the GTPase superfamily, the SRP GTPase exhibits a number of novel properties that we aim to understand at a protein structural level. In addition, we have estabished preliminary conditions for crystallization of the GTP-dependent complex of the SRP GTPase Ffh with its receptor FtsY, also a GTPase. We anticipate optimization of the crystallization conditions in the near future, and are requesting data collection time in order to determine this structure using MAD. The structure of the Ffh/FtsY NG complex is particularly significant, because it will reveal the structural basis for the molecular 'handshake' that plays a central role in SRP-mediated targeting.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这项建议有两个目的：第一个是完成了从Thermus aquaticus的SRP GTfh的GDP和GMPPNP复合体的超高分辨率数据收集。这些结构，与先前在SSRL收集的载脂蛋白晶体的数据，将形成超高分辨率的Ffh结构状态的比较分析的基础。虽然是GTdR超家族的一员，但SRP GTdR表现出许多新的性质，我们的目标是在蛋白质结构水平上了解这些性质。此外，我们已经建立了初步的条件结晶的GTP依赖性复合物的SRP GTfh与其受体FtsY，也是一个GTfh。我们预计在不久的将来优化结晶条件，并要求数据收集时间，以确定使用MAD的这种结构。Ffh/FtsY NG复合物的结构特别重要，因为它将揭示在SRP介导的靶向中起核心作用的分子“握手”的结构基础。