CRYSTALLOGRAPHIC STRUCTURE DETERMINATION OF SYNECHOCYSTIS HEMOGLOBIN PCC 6803

集胞藻血红蛋白 PCC 6803 的晶体结构测定

• 批准号: 7370372
• 负责人: JULIE HOY
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370372
• 关键词: CRYSTALLOGRAPHIC; STRUCTURE; DETERMINATION; SYNECHOCYSTIS; HEMOGLOBIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Truncated hemoglobins (trHb) are a newly discovered family of Hbs with a previously unseen 2-on-2 globin fold resulting in many structural and mechanistic differences compared to typical 3-on-3 globin folds. Synechocystis Hemoglobin PCC 6803 (SynHb) is a trHb with unique ligand binding kinetics. SynHb crystals that diffract to 1.8 angstroms have been used to obtain a native data set and will be used for MAD phasing, necessary due to the absence of a model for molecular replacement. Determination of the SynHb crystal structure will provide the first look at a non-ligand bound, hexacoordinate trHb. This will help to elucidate the complex interaction between distal residues, and provide additional information on the ligand binding properties of SynHb and trHbs in general.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。截断血红蛋白(TrHb)是一个新发现的HBs家族，其2对2珠蛋白折叠与典型的3对3珠蛋白折叠相比有许多结构和机制上的差异。聚球藻血红蛋白PCC6803(SynHb)是一种具有独特的配体结合动力学的trHb。衍射率为1.8埃的SynHb晶体已被用于获得原始数据集，并将用于MAD相移，由于缺乏分子置换模型，这是必要的。SynHb晶体结构的确定将提供第一次看到非配体结合的六配位trHb。这将有助于阐明末端残基之间的复杂相互作用，并提供有关SynHb和trHbs的配体结合性质的更多信息。