REPLICATION COMPLEXES OF RHDV RNA-DEPENDENT RNA POLYMERASE

RHDV RNA 依赖性 RNA 聚合酶的复制复合物

• 批准号: 7370384
• 负责人: Kenneth Ng
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370384
• 关键词: REPLICATION; COMPLEXES; RHDV; RNA; DEPENDENT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rabbit Hemorrhagic disease virus (RHDV) belongs to the Caliciviridae, a family of positive-sense, single-stranded RNA viruses causing serious diseases in mammals, including humans. The viral RNA-dependent RNA polymerase (RdRP) is essential for replicating the viral genome. Hence, understanding the structural and enzymological properties of this enzyme opens avenues for developing antiviral therapeutic agents that interfere with viral replication. Previous work at SSRL has allowed us to solve structures of RHDV RdRP in a number of different conformational states, in complex with different metal ions and in complex with different nucleotides. Preliminary attempts to crystallize and solve structures of complexes with nucleic acid substrates and protein cofactors have led to the identification of a number of new crystal forms. Ongoing biochemical studies, crystallization trials and crystallographic studies are now being carried out to allow for the determination of high-resolution structures of replication complexes of RHDV RdRP.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。兔出血症病毒（Rabbit Hemorrhagic Disease Virus，RHDV）属于杯状病毒科（Caliciviridae），其为正义单链RNA病毒家族，在包括人类在内的哺乳动物中引起严重疾病。病毒RNA依赖性RNA聚合酶（RdRP）是复制病毒基因组所必需的。因此，了解这种酶的结构和酶学性质为开发干扰病毒复制的抗病毒治疗剂开辟了途径。SSRL先前的工作使我们能够解决RHDV RdRP在许多不同构象状态下的结构，与不同金属离子的复合物以及与不同核苷酸的复合物。初步尝试结晶和解决与核酸底物和蛋白质辅因子的复合物的结构，导致了一些新的晶体形式的鉴定。正在进行的生物化学研究，结晶试验和晶体学研究，现在正在进行，以允许确定高分辨率的RHDV RdRP复制复合物的结构。