ROGER KORNBERG PRT TIME

罗杰·科恩伯格 PRT 时间

• 批准号: 7370400
• 负责人: ROGER D KORNBERG
• 金额: $ 1.39万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370400
• 关键词: ROGER; KORNBERG; PRT; TIME

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the proposed research is to determine the X-ray structures of RNA polymerase II and of its complexes with nucleic acids and auxiliary protein factors at atomic resolution. The problem is challenging, since the polymerase alone comprises 15 polypeptides with a total mass of nearly 600,000 Dalton, and addition of the auxiliary factors doubles both the number of polypeptides and the protein mass. Solution of the problem requires a combination of biochemical studies, electron and X-ray crystallography. Eight types of RNA polymerase II crystal are now in hand, crystals of the native enzyme, and cocrystals with DNA, with RNA, with both DNA and RNA in the transcriptionally active state, with TFIIB, with TFIIE, with the additional polymerase subunits RPB4 and RPB7, and with the tight-binding inhibitor a-amanitin. Recent progress includes a low resolution envelope and phases to 6.5 ¿¿ and the recent collection of several novel heavy atom cluster complexed with RNA Pol II. The prognosis is now very good for the rapid achievement of a high resolution structure.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。本研究的目的是在原子分辨率上确定RNA聚合酶II及其与核酸和辅助蛋白因子的复合物的x射线结构。这个问题是具有挑战性的，因为仅聚合酶就包含15个多肽，总质量接近600,000道尔顿，并且添加辅助因子会使多肽的数量和蛋白质质量加倍。解决这个问题需要结合生化研究、电子和x射线晶体学。目前已有八种类型的RNA聚合酶II晶体，包括天然酶的晶体、与DNA、RNA、DNA和RNA均处于转录活性状态的共晶体、与TFIIB、与TFIIE、与附加聚合酶亚基RPB4和RPB7以及与紧密结合抑制剂a-amanitin的共晶体。最近的进展包括低分辨率包膜和6.5¿¿相以及最近收集的几种新型重原子团簇与RNA Pol II络合。现在的预后非常好，可以快速实现高分辨率结构。