ROGER KORNBERG PRT TIME

罗杰·科恩伯格 PRT 时间

• 批准号: 8169914
• 负责人: ROGER D KORNBERG
• 金额: $ 3.15万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169914
• 关键词: Achievement; Amanitins; Binding; Biochemical; Collection; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Polymerase II; Electrons; Enzymes; Funding; Goals; Grant; Hand; Institution; Nucleic Acids; Phase; Polymerase; Proteins; RNA; RNA Polymerase II; Research; Research Personnel; Resolution; Resources; Solutions; Source; Structure; Time; Transcription Factor TFIIB; United States National Institutes of Health; X-Ray Crystallography; dalton; inhibitor/antagonist; novel; outcome forecast; polypeptide; transcription factor TFIIE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the proposed research is to determine the x-ray structures of RNA polymerase II and of its complexes with nucleic acids and auxiliary protein factors at atomic resolution. The problem is challenging, since the polymerase alone comprises 15 polypeptides with a total mass of nearly 600,000 Dalton, and addition of the auxiliary factors doubles both the number of polypeptides and the protein mass. Solution of the problem requires a combination of biochemical studies, electron and X-ray crystallography. Eight types of RNA polymerase II crystal are now in hand, crystals of the native enzyme, and cocrystals with DNA, with RNA, with both DNA and RNA in the transcriptionally active state, with TFIIB, with TFIIE, with the additional polymerase subunits RPB4 and RPB7, and with the tight-binding inhibitor a-amanitin. Recent progress includes a low resolution envelope and phases to 6.5 ¿, and the recent collection of several novel heavy atom cluster complexed with RNA Pol II. The prognosis is now very good for the rapid achievement of a high resolution structure.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 拟议研究的目标是在原子分辨率下确定 RNA 聚合酶 II 及其与核酸和辅助蛋白质因子的复合物的 X 射线结构。这个问题具有挑战性，因为仅聚合酶就包含15个多肽，总质量接近600,000道尔顿，而辅助因子的添加使多肽数量和蛋白质质量都增加了一倍。该问题的解决需要结合生化研究、电子和 X 射线晶体学。目前已有八种类型的 RNA 聚合酶 II 晶体，即天然酶的晶体，以及与 DNA、RNA、DNA 和 RNA 均处于转录活性状态的共晶体、TFIB、TFIIE、附加聚合酶亚基 RPB4 和 RPB7 以及紧密结合抑制剂 α-鹅膏蕈碱的共晶体。最近的进展包括低分辨率包络线和 6.5 ¿ 的相位，以及最近收集的几个与 RNA Pol II 复合的新型重原子簇。现在对于快速实现高分辨率结构的预测非常好。