SIZE CHANGES IN A THERPHILIC-MESOPHILIC PAIR OF DNA POLYMERASES UPON DENATURATIO

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。Doniach及其同事已经使用SSRL BL 4 -2广泛表征了各种蛋白质的变性状态大小，但这将是第一个，或第一个，使用SAXS方法直接测定嗜热-嗜温蛋白质对的变性状态大小之一。这些结构测量将增强对这些蛋白质的变性进行的广泛的热力学测量，这表明这两种蛋白质的变性状态大小应该不同，即使它们的分子量相似。这些结构数据是完成关于这种嗜热-嗜温聚合酶对的比较变性机制的出版物所必需的最后测量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Doniach and associates have used SSRL BL4-2 to extensively characterize denatured state sizes for a wide variety of proteins, but this will be the first, or one of the first, direct determinations of the denatured state sizes for a thermophilic-mesophilic protein pair using SAXS methods. These structural measurements will augment extensive thermodynamic measurements made on the denaturation of these proteins which suggest that the denatured states sizes should differ for the two proteins, even though they are similar in molecular weight. These structural data are the last measurements necessary to complete a publication on the comparative denaturation mechanisms of this thermophilic-mesophilic polymerase pair.