Structural studies of the yeast type II NADH:quinone oxidoreductase NDI-1

酵母 II 型 NADH:醌氧化还原酶 NDI-1 的结构研究

• 批准号: BB/D019311/1
• 负责人: So Iwata
• 金额: $ 56.17万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD019311%2F1
• 关键词: Structural; studies; yeast; type; II

Membrane proteins perform a staggering range of biological functions including respiration, signal transduction and molecular transport. The results of various genome projects have shown that up to 30 % of proteins encoded by eukaryotic cells are membrane proteins. A more fundamental understanding of the structure-function relationships of membrane proteins would make invaluable contributions to structural biology, pharmacology and medicine. Recently, we have obtained well-diffracting crystals of a yeast membrane protein NDI-1. This enzyme is a homologue of the mammalian protein and is an essential enzyme for yeast respiration. Structural information for this membrane protein will provide important insights into this respiratory membrane protein, particularly on its substrate (NADH and coenzyme Q10) and cofactor (FAD) binding sites. The structure is also medically important as it can be used for gene therapy of human genetic diseases.

膜蛋白执行一系列惊人的生物功能，包括呼吸，信号转导和分子运输。各种基因组计划的结果表明，真核细胞编码的蛋白质中高达30%是膜蛋白。对膜蛋白质结构-功能关系的更基本的理解将对结构生物学、药理学和医学做出宝贵的贡献。最近，我们已经获得了良好的衍射晶体的酵母膜蛋白NDI-1。这种酶是哺乳动物蛋白质的同系物，是酵母呼吸的必需酶。这种膜蛋白的结构信息将为这种呼吸膜蛋白提供重要的见解，特别是在其底物（NADH和辅酶Q10）和辅因子（FAD）结合位点上。该结构在医学上也很重要，因为它可以用于人类遗传疾病的基因治疗。