Stuctural studies on human erythrocyte BAND-3

人红细胞BAND-3的结构研究

• 批准号: BB/D019516/1
• 负责人: So Iwata
• 金额: $ 53.48万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD019516%2F1
• 关键词: Stuctural; studies; human; erythrocyte; BAND

The Human Erythrocyte Anion exchanger, AE1, also known as Band 3, allows bicarbonate to cross the red blood cell membrane in exchange for chloride. This is an essential process in allowing the bloodstream to transport large quantities of carbonate to the lungs. Band 3 is also associated with a number of human disease states. In order to understand the precise mechanism of action of the protein and reveal further details on these disease states it is necessary to obtain precise structural information for the human form of Band 3 membrane domain. The most suitable technique for this is X-ray crystallography. The structure determination of human membrane proteins is extremely challenging as evidenced by the fact that no X-ray structure has been solved. So far it has been possible to obtain highly pure Band 3 protein from human red blood cells which has yielded crystals. However the data obtained from the X-ray analysis of the crystals is not of sufficient quality to solve the structure. This application focuses on the optimisation of the Band 3 crystals in order to obtain high quality data suitable for structure determination.

人红细胞阴离子交换剂AE1，也称为带3，允许碳酸氢盐穿过红细胞膜以交换氯化物。这是允许血液将大量碳酸盐输送到肺部的重要过程。带3也与许多人类疾病状态相关。为了了解蛋白质的确切作用机制并揭示这些疾病状态的进一步细节，有必要获得人类形式的带3膜结构域的精确结构信息。最合适的技术是X射线晶体学。人类膜蛋白的结构测定是极具挑战性的，这一事实证明，没有X射线结构已经解决。到目前为止，已经可以从产生晶体的人红细胞中获得高纯度的带3蛋白。然而，从晶体的X射线分析获得的数据的质量不足以解决结构。该应用程序的重点是带3晶体的优化，以获得高质量的数据，适用于结构测定。

项目成果

Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium.

担子菌 Phanerochaete chrysosporium 阴离子交换剂 1 同源物的克隆、表达和纯化。

• DOI: 10.1016/j.pep.2011.04.006
• 发表时间: 2011
• 期刊: Protein expression and purification
• 影响因子: 1.6
• 作者: Tokuda N

Crystal structure of the anion exchanger domain of human erythrocyte band 3

• DOI: 10.1126/science.aaa4335
• 发表时间: 2015-11-06
• 期刊: SCIENCE
• 影响因子: 56.9
• 作者: Arakawa, Takatoshi;Kobayashi-Yurugi, Takami;Iwata, So
• 通讯作者: Iwata, So