Building epithelial cytoarchitecture:regulation of actin dynamics by cell-cell junctions

构建上皮细胞结构：细胞-细胞连接调节肌动蛋白动力学

• 批准号: BB/D019400/1
• 负责人: Vania Braga
• 金额: $ 42.09万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD019400%2F1
• 关键词: Building; epithelial; cytoarchitecture; regulation; actin

Cell-cell adhesion and migration are key events in the life of many different cell types, and are essential for the organization of cells into higher ordered structures such as blood vessels, lung, skin, muscle, etc. Epithelial cells differentiate and form sheets of tightly packed cells: these sheets form protective layers wrapping up the body (skin) and the inside of internal cavities (i.e. stomach, intestines, etc). The choice of whether to migrate or stick to their neighbours has implications for epithelial function in health and disease. Thus, it is important to understand how a cell is kept inside an epithelial sheet or detaches and become shapeless, homeless and aimless. Epithelial cells can not function properly in the absence of tight cell-cell adhesion and a cuboidal morphology. We are interested in understanding how cells glue to their neighbours in order to regulate their shape and differentiation. We aim at identifying the molecules that participate in these processes and investigate their role in epithelia. Insights into how epithelial cell shape is established and maintained will provide clues on how to ameliorate diseases that have compromised epithelial function.

细胞-细胞粘附和迁移是许多不同细胞类型生命中的关键事件，并且对于细胞组织成更高层次的结构如血管、肺、皮肤、肌肉等至关重要。上皮细胞分化并形成紧密堆积的细胞片：这些片形成包裹身体（皮肤）和内腔（即胃、肠等）内部的保护层。选择是迁移还是依附于它们的邻居对健康和疾病中的上皮功能有影响。因此，重要的是要了解细胞是如何保持在上皮层内或分离并变得不成形，无家可归和漫无目的。上皮细胞在缺乏紧密的细胞-细胞粘附和立方形形态的情况下不能正常发挥功能。我们有兴趣了解细胞如何粘附到它们的邻居，以调节它们的形状和分化。我们的目标是确定参与这些过程的分子，并研究它们在上皮细胞中的作用。深入了解上皮细胞形状是如何建立和维持的，将为如何改善上皮功能受损的疾病提供线索。

项目成果

Two-step watershed segmentation of epithelial cells

上皮细胞的两步分水岭分割

• 发表时间: 2011
• 作者: Keraudren, K.

Corrigendum: Defining functional interactions during biogenesis of epithelial junctions.

• DOI: 10.1038/ncomms14195
• 发表时间: 2017-01-05
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Erasmus JC;Bruche S;Pizarro L;Maimari N;Poggioli T;Tomlinson C;Lees J;Zalivina I;Wheeler A;Alberts A;Russo A;Braga VM
• 通讯作者: Braga VM

Editorial Overview: Integration of dynamic processes in cell behaviour and tissue architecture.

编辑概述：细胞行为和组织结构中动态过程的整合。

• DOI: 10.1016/j.ceb.2018.09.005
• 发表时间: 2018
• 期刊: Current opinion in cell biology
• 影响因子: 7.5
• 作者: Braga VM

Cooperation of distinct Rac-dependent pathways to stabilise E-cadherin adhesion.

• DOI: 10.1016/j.cellsig.2015.04.014
• 发表时间: 2015-09
• 期刊: Cellular signalling
• 影响因子: 4.8
• 作者: Erasmus JC;Welsh NJ;Braga VM
• 通讯作者: Braga VM

Defining functional interactions during biogenesis of epithelial junctions.

• DOI: 10.1038/ncomms13542
• 发表时间: 2016-12-06
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Erasmus, J. C.;Bruche, S.;Pizarro, L.;Maimari, N.;Pogglioli, T.;Tomlinson, C.;Lees, J.;Zalivina, I.;Wheeler, A.;Alberts, A.;Russo, A.;Braga, V. M. M.
• 通讯作者: Braga, V. M. M.