Toxicogenimics Laboratory Core

毒理学实验室核心

• 批准号: 7089435
• 负责人: Christine F. Skibola
• 金额: $ 20.67万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7089435
• 关键词: biomedical facility; biotechnology; cell bank /registry; cytogenetics; data collection; environmental toxicology; gene expression; genetic polymorphism; genetic transcription; genetics; mass spectrometry; microarray technology; monitoring device; nanotechnology; proteomics; tissue resource /registry

The goal of this laboratory core is to provide the infrastructure and core expertise for Projects 1-4 to achieve their goals. The overall goal of the Berkeley Superfund Basic Research Program (SBRP) is to apply functional genomics, proteomics, transcriptomics, and nanotechnology to better detect arsenic, mercury, benzene, polycyclic aromatic hydrocarbons, trichloroethylene, and other Superfund priority chemicals in the environment; evaluate their effects on human health, especially the health of susceptible populations such as children; and remediate their presence and reduce their toxicity. Projects 1 through 4 will use functional genomics, proteomics, and transcriptomics in their studies. Further, Projects 1 and 3 are epidemiological studies that require sophisticated sample processing so that these technologies can be applied. The success of Projects 1- 4 largely depends on the effective handling and management of biological samples, as well as access to and expertise in the latest "-omic" technologies. Thus, detailed collection and storage protocols have been designed and core facilities will be provided for the cytogenetic, genotyping, gene expression and proteomic analyses proposed in Projects 1-4. The specific aims of Core C are to: 1) Process, maintain and store biological samples and cell lines; 2) provide facilities and methodologies for cytogenetic analysis; 3) provide facilities for gene expression profiling using Affymetrix, Illumina, and custom array technologies; 4) provide facilities for proteomic analyses using various mass spectrometric technologies; and, 5) provide facilities and methodologies for the analysis of genetic polymorphisms by Taqman-based and bead array technologies using the ABI 7900 Sequence Detection System and Illumina Bead Station platforms. Expertise in all required areas has been attained that, along with the computational biology skills of Core D, should allow the successful application of "-omic" technologies in Projects 1-4 of the Berkeley SBRP.

该实验室核心的目标是提供基础设施 和项目1-4实现目标的核心专业知识。伯克利超级基金的总体目标 基础研究计划（SBRP）是应用功能基因组学，蛋白质组学，转录组学和 纳米技术可以更好地检测砷，汞，苯，多环芳烃， 环境中的三氯乙烯和其他超级基金优先级化学品；评估他们对 人类健康，尤其是易感人群的健康，例如儿童；并补充他们的 存在并降低其毒性。项目1至4将使用功能基因组学，蛋白质组学和 转录组学在他们的研究中。此外，项目1和3是需要的流行病学研究 复杂的样品处理，以便可以应用这些技术。项目的成功1- 4很大程度上取决于生物样品的有效处理和管理以及访问 在最新的“ - 组”技术中进行专业知识。因此，详细的收集和存储协议具有 已设计并为细胞遗传学，基因分型，基因表达和 项目1-4提出的蛋白质组学分析。核心C的具体目的是：1）处理，维护 并存储生物样品和细胞系； 2）提供细胞遗传学的设施和方法论 分析; 3）提供使用Affymetrix，Illumina和自定义阵列的基因表达分析的设施 技术； 4）提供使用各种质谱法的蛋白质组学分析的设施 技术； 5）提供了通过分析遗传多态性的设施和方法 使用ABI 7900序列检测系统和基于TAQMAN的珠阵列技术和 Illumina珠子站平台。已经达到了所有必需领域的专业知识， 核心D的计算生物学技能应允许成功应用“ - 摩尼克”技术 在伯克利SBRP的1-4项目中。