Core--Functional Geomics and Bioinformatics
核心--功能基因组学与生物信息学
基本信息
- 批准号:7089381
- 负责人:
- 金额:$ 46.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-04-01 至 2009-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Research from various laboratories, including those of SBRP investigators, has established that in vivo levels of particular biotransformation enzymes are relevant to numerous toxicological issues. For example, genetic correlates have been identified in several molecular epidemiologic investigations between particular polymorphisms in biotransformation genes and the incidence of environmental-related disease. In addition, biological monitoring has increasingly involved the assessment of genetic/molecular indices from individuals and sentinel animals, for the purpose of detecting possible markers of disease susceptibility, as well as identification of early indicators of chemical effect - such as alterations in biotransformation enzyme gene expression profiles due to exposure to environmental toxicants. Advances in technology supporting
molecular research have led to the development of complex techniques involving these biotransformation enzyme systems, which are applicable to a broad range of experimental problems including novel remediation strategies. The long term objective of the UW SBRP Functional Genomics and Bioinformatics Core C Laboratory is to provide a service facility for UW SBRP investigators enabling novel genetic assay development, high throughput genetic and proteomic analysis, state-of-the-art quantitative gene expression and bioinformatic technologies, and correlates to environmental-related disease mechanisms. All six individual UW SBRP projects will utilize the UW SBRP Core C Laboratory and will benefit from the centralized services. These Core efforts will focus on a wide range of molecular biology- and proteomic-related technologies that may be predictive of: exposure to toxicants; impaired physiologic and neurologic function; unusual susceptibility to neurological damage from toxic agents that occur in the environment, particularly those that are commonly present at hazardous waste sites. Over the 5 year duration of this proposal, the UW SBRP Core C Laboratory will provide the following services: DNA/RNA extraction of 1,200 samples, the isolation of 625 mononuclear cell samples, processing and bioinformatic analyses of 255 GeneChips and 98 cDNA salmonoid arrays, the production of a custom "SNP chip" representing up to 2,500
genetic variants and subsequent processing and genetic association analyses, the identification of up to approximately 5,000,000 genotypes, the development of 25 new TaqMan-based genotyping assays, the construction of 131 new gene expression assays, the execution of approximately 7,800 gene expression analyses, the provision of approximately 4,000 DNA sequencing gel lanes, the generation of 10 peptide derived antibodies, and the creation of 15
novel ELISAs. This shared resource provided by the UW SBRP Core C Laboratory supports implementation of the requisite functional genomic assays in a cost-effective and efficient manner, therefore maximizing the availability of these important tools for UW SBRP investigators.
来自不同实验室的研究,包括SBRP研究人员的研究,已经确定特定生物转化酶的体内水平与许多毒理学问题相关。例如,在一些分子流行病学调查中,已经确定了生物转化基因中特定多态性与环境相关疾病发病率之间的遗传相关性。 此外,生物监测越来越多地涉及对个人和哨兵动物的遗传/分子指数进行评估,以检测疾病易感性的可能标志,并确定化学效应的早期指标-例如由于接触环境毒物而导致的生物转化酶基因表达谱的改变。技术支持方面的进展
分子研究导致了涉及这些生物转化酶系统的复杂技术的发展,这些技术适用于包括新的补救策略在内的广泛的实验问题。UW SBRP功能基因组学和生物信息学核心C实验室的长期目标是为UW SBRP研究人员提供服务设施,使新的遗传检测开发,高通量遗传和蛋白质组学分析,最先进的定量基因表达和生物信息学技术,并与环境相关的疾病机制相关。所有六个单独的UW SBRP项目将利用UW SBRP核心C实验室,并将受益于集中服务。这些核心工作将侧重于广泛的分子生物学和蛋白质组学相关技术,这些技术可能预测:接触有毒物质;生理和神经功能受损;对环境中出现的有毒物质造成的神经损伤的异常敏感性,特别是那些通常存在于危险废物场地的有毒物质。在该提案的5年期间,UW SBRP核心C实验室将提供以下服务:1,200个样本的DNA/RNA提取,625个单核细胞样本的分离,255个基因芯片和98个cDNA鲑鱼阵列的处理和生物信息学分析,代表多达2,500个基因的定制“SNP芯片”的生产。
遗传变异和随后的处理和遗传关联分析,鉴定多达约5,000,000种基因型,开发25种新的基于TaqMan的基因分型测定,构建131种新的基因表达测定,执行约7,800种基因表达分析,提供约4,000条DNA测序凝胶泳道,产生10种肽衍生抗体,并产生15种
小说ELISA。UW SBRP核心C实验室提供的这种共享资源支持以具有成本效益和高效的方式实施必要的功能基因组测定,从而最大限度地提高UW SBRP研究人员这些重要工具的可用性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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FERDERICO M FARIN其他文献
FERDERICO M FARIN的其他文献
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{{ truncateString('FERDERICO M FARIN', 18)}}的其他基金
Research Support Core C: Functional Genomics and Bioinformatics Core
研究支持核心 C:功能基因组学和生物信息学核心
- 批准号:
8845303 - 财政年份:2014
- 资助金额:
$ 46.38万 - 项目类别:
Research Support Core C: Functional Genomics and Bioinformatics Core
研究支持核心 C:功能基因组学和生物信息学核心
- 批准号:
8377599 - 财政年份:2012
- 资助金额:
$ 46.38万 - 项目类别:
Research Support Core C: Functional Genomics and Bioinformatics Core
研究支持核心 C:功能基因组学和生物信息学核心
- 批准号:
8254491 - 财政年份:2011
- 资助金额:
$ 46.38万 - 项目类别:
Research Support Core C: Functional Genomics and Bioinformatics Core
研究支持核心 C:功能基因组学和生物信息学核心
- 批准号:
8065493 - 财政年份:2010
- 资助金额:
$ 46.38万 - 项目类别:
Research Support Core C: Functional Genomics and Bioinformatics Core
研究支持核心 C:功能基因组学和生物信息学核心
- 批准号:
7622922 - 财政年份:2009
- 资助金额:
$ 46.38万 - 项目类别: