Molecular Characterization of Parthenogenetic Meiosis

孤雌减数分裂的分子特征

• 批准号: 7386549
• 负责人: DAYALAN G SRINIVASAN
• 金额: $ 5.2万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7386549
• 关键词: Alleles; Animals; Aphids; Behavior; Biological; Biological Models; Candidate Disease Gene; Cells; Disease; Environment; Evolution; Exhibits; Genetic Recombination; Genomics; Goals; Meiosis; Meiotic Recombination; Modification; Molecular; Molecular Genetics; Pathway interactions; Personal Satisfaction; Physiological; Pisum; Pisum sativum; Plastics; Process; Proteins; Reproduction; Research; Testing; Tissue-Specific Gene Expression; asexual; cDNA Arrays; inhibitor/antagonist; positional cloning; reproductive; research study; response; small molecule

Phenotypic plasticity, the expression of alternate physiological states, morphological forms and/or distinct behaviors in response to specific environments, is a universal phenomenon in animals. Although the evolution of plastic responses is well understood theoretically, little is known about the genetic and molecular mechanisms of this response and the importance of plasticity in disease. The pea aphid Acyrthosiphon pisum, an emerging model system, exhibits an adaptive plasticity in which environmental modifications to meiosis result in parthenogenetic clonal reproduction. How aphids have evolved plasticity in this fundamental process that promotes recombination and reassortment of alleles is unknown. The goal of the proposed research is to understand meiotic plasticity at the molecular level. I propose to examine the discrete molecular differences that produce sexual and asexual meiosis in aphids using cell biological and genomic approaches. Candidate genes and pathways identified by these experiments will be functionally tested using reverse genetics and small molecule inhibitors. These experiments represent the first step toward a molecular characterization of a dramatic example of adaptive plasticity in an ancient, highly conserved process.

表型可塑性，交替生理状态的表达，形态形式和/或 在特定的环境中，不同的行为是动物的普遍现象。虽然 塑性反应的进化在理论上是很好理解的，但对遗传和 这种反应的分子机制和可塑性在疾病中的重要性。豌豆蚜虫 豌豆蚜是一个新出现的模式系统，具有适应性可塑性， 对减数分裂的修饰导致孤雌生殖克隆繁殖。蚜虫如何进化 在这个促进等位基因重组和重配的基本过程中， 未知这项研究的目的是在分子水平上了解减数分裂的可塑性。我 建议检查产生有性和无性减数分裂的离散分子差异， 利用细胞生物学和基因组学方法研究蚜虫候选基因和途径鉴定 这些实验将使用反向遗传学和小分子抑制剂进行功能测试。 这些实验代表了对一个戏剧性的例子进行分子表征的第一步， 适应性可塑性在一个古老的，高度保守的过程。

项目成果

Chromatin-remodelling proteins of the pea aphid, Acyrthosiphon pisum (Harris).

• DOI: 10.1111/j.1365-2583.2009.00972.x
• 发表时间: 2010-03
• 期刊: Insect molecular biology
• 影响因子: 2.6
• 作者: Rider SD Jr;Srinivasan DG;Hilgarth RS
• 通讯作者: Hilgarth RS

Gene expression analysis of parthenogenetic embryonic development of the pea aphid, Acyrthosiphon pisum, suggests that aphid parthenogenesis evolved from meiotic oogenesis.

• DOI: 10.1371/journal.pone.0115099
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Srinivasan DG;Abdelhady A;Stern DL
• 通讯作者: Stern DL