Gene expression analysis of parthenogenetic embryonic development of the pea aphid, Acyrthosiphon pisum, suggests that aphid parthenogenesis evolved from meiotic oogenesis.
Gene expression analysis of parthenogenetic embryonic development of the pea aphid, Acyrthosiphon pisum, suggests that aphid parthenogenesis evolved from meiotic oogenesis.
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DOI：
10.1371/journal.pone.0115099
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Stern DL
中科院分区：
文献类型：
--
作者：
Srinivasan DG;Abdelhady A;Stern DL
蚜虫表现出一种表型可塑性，称为多物性，即遗传相同的雌性在生命周期的一部分有性繁殖，在生命周期的其余部分无性繁殖(通过孤雌生殖)。蚜虫孤雌生殖的分子基础尚不清楚。对蚜虫孤雌生殖的细胞学观察表明，无性卵子发生要么是通过减数分裂的修饰，要么是从有丝分裂过程进化而来的。作为对这些选择的测试，我们评估了典型的减数分裂重组和生殖系基因在豌豆蚜有性和无性子房中的表达水平和表达模式。我们观察到所有减数分裂基因在无性卵巢和有性卵巢中的表达模式相似，除了一些编码Argavite家族成员的基因在有性卵巢中不表达外。此外，我们还观察到，无性蚜虫组织积累了Spo11的非剪接转录本，而有性蚜虫组织积累了主要的剪接转录本。原位杂交显示Spo11在有性生殖细胞中表达，在无性生殖细胞中检测不到。我们还发现，一个专性无性品系的豌豆蚜能产生很少的剪接Spo11转录本。综上所述，这些结果表明，孤雌生殖卵子发生是从类似减数分裂的过程而不是类似有丝分裂的过程进化而来的，并且蚜虫的生殖多物性可能涉及Spo11基因活性的修饰。
Aphids exhibit a form of phenotypic plasticity, called polyphenism, in which genetically identical females reproduce sexually during one part of the life cycle and asexually (via parthenogenesis) during the remainder of the life cycle. The molecular basis for aphid parthenogenesis is unknown. Cytological observations of aphid parthenogenesis suggest that asexual oogenesis evolved either through a modification of meiosis or from a mitotic process. As a test of these alternatives, we assessed the expression levels and expression patterns of canonical meiotic recombination and germline genes in the sexual and asexual ovaries of the pea aphid, Acyrthosiphon pisum. We observed expression of all meiosis genes in similar patterns in asexual and sexual ovaries, with the exception that some genes encoding Argonaute-family members were not expressed in sexual ovaries. In addition, we observed that asexual aphid tissues accumulated unspliced transcripts of Spo11, whereas sexual aphid tissues accumulated primarily spliced transcripts. In situ hybridization revealed Spo11 transcript in sexual germ cells and undetectable levels of Spo11 transcript in asexual germ cells. We also found that an obligately asexual strain of pea aphid produced little spliced Spo11 transcript. Together, these results suggest that parthenogenetic oogenesis evolved from a meiosis-like, and not a mitosis-like, process and that the aphid reproductive polyphenism may involve a modification of Spo11 gene activity.
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影响因子：
56.9
作者：
Lin, Yanfeng;Gill, Mark E.;Page, David C.
通讯作者：
Page, David C.
影响因子：
3.6
作者：
Kehoe, Sarah M.;Oka, Masahiro;Terada, Naohiro
通讯作者：
Terada, Naohiro
影响因子：
10.5
作者：
McKim, KS;Hayashi-Hagihara, A
通讯作者：
Hayashi-Hagihara, A
影响因子：
1.5
作者：
Hales, DF;Wilson, ACC;Sunnucks, P
通讯作者：
Sunnucks, P
DOI：
10.1016/s0020-7322(78)80013-0
发表时间：
1978-01-01
期刊：
INTERNATIONAL JOURNAL OF INSECT MORPHOLOGY & EMBRYOLOGY
影响因子：
--
作者：
BLACKMAN, RL
通讯作者：
BLACKMAN, RL