Location function and structural properties of the Nhm1 nuclear decapping enzyme in fission yeast

裂殖酵母 Nhm1 核脱帽酶的定位功能和结构特性

• 批准号: BB/E00640X/1
• 负责人: John McCarthy
• 金额: $ 45.46万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE00640X%2F1
• 关键词: Location; function; structural; properties; Nhm1

The information contained in the genes of living cells has to be converted into cellular components that form structures and enable biochemical reactions to take place. This process is called gene expression and it is vital to all life. Gene expression comprises two main steps, called transcription and translation. In transcription, the information in the DNA sequences of the genes is converted into equivalent sequences in so-called messenger RNA (mRNA) molecules. In translation, the mRNA molecules are 'read' by a large molecular structure called the ribosome, which uses the information to dictate the synthesis of proteins. Each cell has to synthesize and break down its mRNA molecules, because this continuous turnover allows the cell to adapt its patterns of gene expression more rapidly to changes in the demand for particular proteins which are needed under certain growth conditions. Our work will improve understanding of an important enzyme that is involved in the cellular metabolism of such RNA molecules. We intend to use methods of structural biology, biochemistry and microscopy to work out where in the cell this enzyme works, how it chooses the RNA molecules it cleaves on the basis of their length, and how its structure enables it to work efficiently as an enzyme.

活细胞基因中所含的信息必须转化为细胞成分，形成结构，使生化反应能够发生。这个过程被称为基因表达，它对所有生命都至关重要。基因表达包括两个主要步骤，称为转录和翻译。在转录过程中，基因的DNA序列中的信息被转换成所谓信使RNA（mRNA）分子中的等同序列。在翻译过程中，mRNA分子被称为核糖体的大分子结构“读取”，核糖体使用信息来决定蛋白质的合成。每个细胞都必须合成和分解其mRNA分子，因为这种连续的周转使细胞能够更快地适应其基因表达模式的变化，以适应特定生长条件下所需的特定蛋白质的需求。我们的工作将提高对参与这种RNA分子的细胞代谢的重要酶的理解。我们打算使用结构生物学、生物化学和显微镜的方法来研究这种酶在细胞中的何处起作用，它如何根据长度选择它切割的RNA分子，以及它的结构如何使它作为一种酶有效地工作。

项目成果

Found in translation: another RNA helicase function.

翻译中发现：另一种RNA解旋酶功能。

• DOI: 10.1016/j.molcel.2008.12.008
• 发表时间: 2008
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Stevenson AL