GARLIC METABOLISM AND CYTOCHROME P450 MODULATION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。人们越来越关注在柜台上销售的许多草药产品的质量、有效性和安全性。一个主要的安全问题是草药和药物之间潜在的不良相互作用。2002年，大蒜补充剂是美国最畅销的草药产品。本研究的总体目标是研究四种常用的大蒜补充剂，即高、低含量的大蒜粉、大蒜油和陈化大蒜对健康受试者几种主要药物代谢细胞色素P450酶(Cyps)和膜外流转运蛋白P-糖蛋白(PGP)活性的调节作用。在每种大蒜制剂的急性和慢性处理后，将使用CYP和PGP特异性药物底物探针的“鸡尾酒”来测量CyPS和Pgp的体内活性。这些探测研究产生的信息将使我们能够预测大蒜补充剂和处方药之间的潜在相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is growing concern over the quality, efficacy and safety of the many herbal products sold over the counter. A major safety concern is the potential of adverse interactions between herbs and drugs. In 2002, garlic supplements were the top selling herbal product in the U. S. The overall objective of this proposed research is to investigate the modulating effects of four commonly used garlic supplements, i.e., garlic powders with high and low allicin content, garlic oil and aged garlic, on the activity of several major drug-metabolizing cytochrome P450 enzymes (CYPs) and the membrance efflux transporter P-glycoprotein (Pgp) in healthy subjects. In vivo activity of CYPs and Pgp will be measured using "cocktails" of CYP and Pgp-specific drug substrate probes following acute and chronic treatment of each garlic preparation. Information generated from these probe studies will allow us to predict potential interactions between garlic supplements and prescription drugs.